Bex1 knock out mice show altered skeletal muscle regeneration

Bex1 and Calmodulin (CaM) are upregulated during skeletal muscle regeneration. We confirm this finding and demonstrate the novel finding that they interact in a calcium-dependent manner. To study the role of Bex1 and its interaction with CaM in skeletal muscle regeneration, we generated Bex1 knock o...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-11, Vol.363 (2), p.405-410
Main Authors: Koo, Jae Hyung, Smiley, Mark A., Lovering, Richard M., Margolis, Frank L.
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Animals
Bex1
Bex1-KO mice
CALCIUM
CALCIUM IONS
CALMODULIN
CaM-binding protein
CELL DIFFERENTIATION
CELL PROLIFERATION
EXERCISE
INTRAMUSCULAR INJECTION
Male
MICE
Mice, Knockout
Muscle Fatigue - physiology
Muscle regeneration
Muscle, Skeletal - physiology
MUSCLES
Myogenesis
Nerve Tissue Proteins - physiology
Omnibank
Physical Exertion - physiology
REGENERATION
Regeneration - physiology
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Summary:Bex1 and Calmodulin (CaM) are upregulated during skeletal muscle regeneration. We confirm this finding and demonstrate the novel finding that they interact in a calcium-dependent manner. To study the role of Bex1 and its interaction with CaM in skeletal muscle regeneration, we generated Bex1 knock out (Bex1-KO) mice. These mice appeared to develop normally and are fertile, but displayed a functional deficit in exercise performance compared to wild type (WT) mice. After intramuscular injection of cardiotoxin, which causes extensive and reproducible myotrauma followed by recovery, regenerating muscles of Bex1-KO mice exhibited elevated and prolonged cell proliferation, as well as delayed cell differentiation, compared to WT mice. Thus, our results provide the first evidence that Bex1-KO mice show altered muscle regeneration, and allow us to propose that the interaction of Bex1 with Ca 2+/CaM may be involved in skeletal muscle regeneration.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.08.186