Distribution of mip-related sequences in 39 species (48 serogroups) of Legionellaceae

The macrophage infectivity potentiator gene (mip) from Legionella pneumophila is a major virulence factor of the species. Thus, mip-detection by amplification has been proposed to assess the presence of L. pneumophila in clinical and environmental samples. The distribution of mip-related sequences w...

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Bibliographic Details
Published in:Epidemiology and infection 1996-12, Vol.117 (3), p.501-506
Main Authors: Riffard, S., Vandenesch, F., Reyrolle, M., Etienne, J.
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Blotting, Southern
DNA
DNA probes
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Gels
Gene Amplification
Genetic hybridization
Genetics
Legionella
Legionella pneumophila
Legionellaceae
Legionellaceae - classification
Legionellaceae - genetics
Legionellaceae - isolation & purification
Macrophage Inflammatory Proteins - genetics
Microbiology
Polymerase Chain Reaction
Sequencing
Signal amplification
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Summary:The macrophage infectivity potentiator gene (mip) from Legionella pneumophila is a major virulence factor of the species. Thus, mip-detection by amplification has been proposed to assess the presence of L. pneumophila in clinical and environmental samples. The distribution of mip-related sequences within the Legionellaceae was studied by DNA amplification using mip-specific primers followed by Southern blot hybridization with an internal probe. Thirty nine species (48 serogroups) of Legionellaceae were screened in this attempt. Using this approach, sequences related to mip were observed in 89% of the tested species including the most recently described L. fairfieldensis, L. lansingensis and L. shakespearei. In several cases, cloning and sequencing of the amplified products confirmed the high levels of similarity between the sequence found in non-pneumophila species with that of the L. pneumophila mip gene. This confirms previous reports that mip related genes are widespread among Legionellaceae and therefore specific detection of the species L. pneumophila cannot be based on mip-targeted amplification.
ISSN:0950-2688
1469-4409
DOI:10.1017/S0950268800059173