Identification of human and bovine rotavirus serotypes by polymerase chain reaction

The use of the polymerase chain reaction (PCR) for identifying serotypes of human and bovine rotaviruses was examined. In the identification of 115 human rotavirus samples in stools, results with PCR showed excellent agreement with results of serotyping by an enzyme-linked immunosorbent assay (ELISA...

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Bibliographic Details
Published in:Epidemiology and infection 1992-10, Vol.109 (2), p.303-312
Main Authors: Taniguchi, K., Wakasugi, F., Pongsuwanna, Y., Urasawa, T., Ukae, S., Chiba, S., Urasawa, S.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Base Sequence
Biological and medical sciences
Cattle
DNA
Enzyme linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - standards
Epitopes
Evaluation Studies as Topic
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Molecular Sequence Data
Monoclonal antibodies
Polymerase Chain Reaction
Rotavirus
Rotavirus - classification
Rotavirus - genetics
Rotavirus Infections - epidemiology
Rotavirus Infections - microbiology
Sensitivity and Specificity
Serotyping
Serotyping - methods
Serotyping - standards
Techniques used in virology
Ungulates
Virology
Viruses
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Summary:The use of the polymerase chain reaction (PCR) for identifying serotypes of human and bovine rotaviruses was examined. In the identification of 115 human rotavirus samples in stools, results with PCR showed excellent agreement with results of serotyping by an enzyme-linked immunosorbent assay (ELISA) using serotype-specific monoclonal antibodies. Furthermore, the PCR showed a much higher sensitivity (93%) than the ELISA test (82·6%). The PCR method could also be applied for identifying the serotype of bovine rotaviruses.
ISSN:0950-2688
1469-4409
DOI:10.1017/S0950268800050263