Genetic architecture of mitochondrial editing in Arabidopsis thaliana

• Published in: Genetics (Austin) 2008-03, Vol.178 (3), p.1693-1708
• Main Authors: Bentolila, S, Elliott, L.E, Hanson, M.R
• Format: Article
• Language: English
• Subjects: additive gene effects; Arabidopsis - genetics; Arabidopsis thaliana; Base Sequence; Chromosome Mapping; Crosses, Genetic; dominance (genetics); E coli; Gene Expression Profiling; Genes; Genes, Plant; Genetics; Inbreeding; Investigations; messenger RNA; Mitochondria - genetics; mitochondrial DNA; Molecular Sequence Data; nucleotide sequences; Phenotype; Plant Leaves - genetics; Polymorphism, Single Nucleotide - genetics; Proteins; quantitative trait loci; Quantitative Trait Loci - genetics; Quantitative Trait, Heritable; RNA editing; RNA Editing - genetics; Sequence Alignment; Sequence Analysis, DNA; single nucleotide polymorphism; transcriptome
• ISSN: 0016-6731; 1943-2631; 1943-2631
• DOI: 10.1534/genetics.107.073585

We have analyzed the mitochondrial editing behavior of two Arabidopsis thaliana accessions, Landsberg erecta (Ler) and Columbia (Col). A survey of 362 C-to-U editing sites in 33 mitochondrial genes was conducted on RNA extracted from rosette leaves. We detected 67 new editing events in A. thaliana rosette leaves that had not been observed in a prior study of mitochondrial editing in suspension cultures. Furthermore, 37 of the 441 C-to-U editing events reported in A. thaliana suspension cultures were not observed in rosette leaves. Forty editing sites that are polymorphic in extent of editing were detected between Col and Ler. Silent editing sites, which do not change the encoded amino acid, were found in a large excess compared to nonsilent sites among the editing events that differed between accessions and between tissue types. Dominance relationships were assessed for 15 of the most polymorphic sites by evaluating the editing values of the reciprocal hybrids. Dominance is more common in nonsilent sites than in silent sites, while additivity was observed only in silent sites. A maternal effect was detected for 8 sites. QTL mapping with recombinant inbred lines detected 12 major QTL for 11 of the 13 editing traits analyzed, demonstrating that efficiency of editing of individual mitochondrial C targets is generally governed by a major factor.