Angiotensin II-acetylcholine noncovalent complexes analyzed with MALDI-ion mobility-TOF MS

Matrix-assisted laser desorption ionization-ion mobility-orthogonal time-of-flight mass spectrometry (MALDI-IM oTOF MS) is a new technique that allows laser desorbed ion to be preseparated on the basis of their shape prior to mas analysis. Using this instrument, we tested the postulate that addition...

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Bibliographic Details
Published in:Journal of biomolecular techniques 2003-03, Vol.14 (1), p.1-8
Main Authors: Woods, Amina S, Fuhrer, Katrin, Gonin, Marc, Egan, Tom, Ugarov, Michael, Gillig, Kent J, Schultz, J Albert
Format: Article
Language:English
Subjects:
Acetylcholine - chemistry
Angiotensin II - chemistry
Macromolecular Substances
Phosphorylation
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Thymine - analogs & derivatives
Thymine - chemistry
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Summary:Matrix-assisted laser desorption ionization-ion mobility-orthogonal time-of-flight mass spectrometry (MALDI-IM oTOF MS) is a new technique that allows laser desorbed ion to be preseparated on the basis of their shape prior to mas analysis. Using this instrument, we tested the postulate that addition of a quaternary ammonium compound such as acetylcholine to the model phosphorylated peptide angio tensin II would enhance its detection by MALDI in two ways. First of all, the acetylcholine-peptide complex could ionize more efficiently than the bare phosphopeptide. Furthermore the ion mobility could separate the complex ion on the basis of its charge/volume from isobaric interferences, which would otherwise limit detection sensitivity.
ISSN:1524-0215