Genetically modified yolk proteins precipitate in the adult Drosophila fat body

Ultrastructural and genetic studies were carried out on the fat body of a female sterile mutant fs(1)1163 to ascertain why yolk protein 1 (YP1) is not secreted from this tissue. Earlier molecular studies demonstrated that (a) normally yolk protein is synthesized in the fat body, secreted into the he...

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Bibliographic Details
Published in:The Journal of cell biology 1991-02, Vol.112 (4), p.727-737
Main Authors: Butterworth, F.M. (Oakland University, Rockester, MI), Bownes, M, Burde, V.S
Format: Article
Language:English
Subjects:
Animals
APPAREIL DIGESTIF
Basement membrane
Basement Membrane - metabolism
Basement Membrane - ultrastructure
Biochemistry. Physiology. Immunology
Biological and medical sciences
Cell membranes
Cells
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
DROSOPHILA
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Egg proteins
Egg Proteins - genetics
Egg Proteins - metabolism
ESTRUCTURA CELULAR
Fat body
Fat Body - metabolism
Fat Body - ultrastructure
Female
Fundamental and applied biological sciences. Psychology
Genes
GENOTIPOS
GENOTYPE
Golgi apparatus
identification
Insecta
Invertebrates
LIPIDE
LIPIDOS
Lysosomes
Materials
Molecular Sequence Data
MUTANT
MUTANTES
Mutation
Physiology. Development
PROTEINAS
PROTEINE
SISTEMA DIGESTIVO
STRUCTURE CELLULAIRE
VARIACION GENETICA
VARIATION GENETIQUE
yolk protein 1
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Description
Summary:Ultrastructural and genetic studies were carried out on the fat body of a female sterile mutant fs(1)1163 to ascertain why yolk protein 1 (YP1) is not secreted from this tissue. Earlier molecular studies demonstrated that (a) normally yolk protein is synthesized in the fat body, secreted into the hemolymph and taken up by the ovary, (b) the 1163 mutation causes a single amino acid substitution in YP1, and (c) females homozygous for the mutation, or heterozygous females raised at 29 degrees C, retain YP1 in the fat body. Ultrastructural analysis in this paper shows that the fat body of these females contains masses of electron-dense material deposited in the subbasement membrane space. This subbasement membrane material (SBMM), which occasionally has a crystalline-like, fibrous component, is found in females whose genotypes include at least one copy of the mutant 1163 gene. These strains include a deletion strain that is hemizygous for the 1163 gene and two strains that are transgenic for the mutant gene. Immunogold studies indicate that SBMM contains yolk protein. We propose that the mutant protein is secreted into the subbasement membrane space, but because of the amino acid substitution in YP1, the oligomers containing YP1 condense into SBMM, which cannot penetrate the basement membrane. The similarity of SBMM and deoxyhemoglobin S fibers is discussed
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.112.4.727