Assembly of the Mammalian Muscle Acetylcholine Receptor in Transfected COS Cells

We have investigated the mechanisms of assembly and transport to the cell surface of the mouse muscle nicotinic acetylcholine receptor (AChR) in transiently transfected COS cells. In cells transfected with all four subunit cDNAs, AChR was expressed on the surface with properties resembling those see...

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Bibliographic Details
Published in:The Journal of cell biology 1991-08, Vol.114 (4), p.799-807
Main Authors: Gu, Yong, Forsayeth, John R., Verrall, Shahla, Yu, Xiao Mei, Hall, Zach W.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Monoclonal
Biological and medical sciences
Bungarotoxins - metabolism
Cell Line
Cell Membrane - metabolism
Cell receptors
Cell structures and functions
Cells
Cholinergic receptors
Complementary DNA
COS cells
Fundamental and applied biological sciences. Psychology
Genetic Vectors
Immunoprecipitation
Kinetics
Macromolecular Substances
Mice
Molecular and cellular biology
Molecular Weight
Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine)
Muscles - physiology
Neurons
Oligomers
Receptors
Receptors, Cholinergic - biosynthesis
Receptors, Cholinergic - genetics
Receptors, Cholinergic - metabolism
skeletal muscle
Transfection
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Summary:We have investigated the mechanisms of assembly and transport to the cell surface of the mouse muscle nicotinic acetylcholine receptor (AChR) in transiently transfected COS cells. In cells transfected with all four subunit cDNAs, AChR was expressed on the surface with properties resembling those seen in mouse muscle cells. When incomplete combinations of AChR subunits were expressed, surface binding of 125I-α-bungarotoxin was not detected except in the case of αβγ which expressed
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.114.4.799