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Inhibition of the active lymph pump by flow in rat mesenteric lymphatics and thoracic duct
There are only a few reports of the influence of imposed flow on an active lymph pump under conditions of controlled intraluminal pressure. Thus, the mechanisms are not clearly defined. Rat mesenteric lymphatics and thoracic ducts were isolated, cannulated and pressurized. Input and output pressures...
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Published in: | The Journal of physiology 2002-05, Vol.540 (3), p.1023-1037 |
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description | There are only a few reports of the influence of imposed flow on an active lymph pump under conditions of controlled intraluminal
pressure. Thus, the mechanisms are not clearly defined. Rat mesenteric lymphatics and thoracic ducts were isolated, cannulated
and pressurized. Input and output pressures were adjusted to impose various flows. Lymphatic systolic and diastolic diameters
were measured and used to determine contraction frequency and pump flow indices. Imposed flow inhibited the active lymph pump
in both mesenteric lymphatics and in the thoracic duct. The active pump of the thoracic duct appeared more sensitive to flow
than did the active pump of the mesenteric lymphatics. Imposed flow reduced the frequency and amplitude of the contractions
and accordingly the active pump flow. Flow-induced inhibition of the active lymph pump followed two temporal patterns. The
first pattern was a rapidly developing inhibition of contraction frequency. Upon imposition of flow, the contraction frequency
immediately fell and then partially recovered over time during continued flow. This effect was dependent on the magnitude
of imposed flow, but did not depend on the direction of flow. The effect also depended upon the rate of change in the direction
of flow. The second pattern was a slowly developing reduction of the amplitude of the lymphatic contractions, which increased
over time during continued flow. The inhibition of contraction amplitude was dependent on the direction of the imposed flow,
but independent of the magnitude of flow. Nitric oxide was partly but not completely responsible for the influence of flow
on the mesenteric lymph pump. Exposure to NO mimicked the effects of flow, and inhibition of the NO synthase by N G -monomethyl- l -arginine attenuated but did not completely abolish the effects of flow. |
doi_str_mv | 10.1113/jphysiol.2001.016642 |
format | article |
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pressure. Thus, the mechanisms are not clearly defined. Rat mesenteric lymphatics and thoracic ducts were isolated, cannulated
and pressurized. Input and output pressures were adjusted to impose various flows. Lymphatic systolic and diastolic diameters
were measured and used to determine contraction frequency and pump flow indices. Imposed flow inhibited the active lymph pump
in both mesenteric lymphatics and in the thoracic duct. The active pump of the thoracic duct appeared more sensitive to flow
than did the active pump of the mesenteric lymphatics. Imposed flow reduced the frequency and amplitude of the contractions
and accordingly the active pump flow. Flow-induced inhibition of the active lymph pump followed two temporal patterns. The
first pattern was a rapidly developing inhibition of contraction frequency. Upon imposition of flow, the contraction frequency
immediately fell and then partially recovered over time during continued flow. This effect was dependent on the magnitude
of imposed flow, but did not depend on the direction of flow. The effect also depended upon the rate of change in the direction
of flow. The second pattern was a slowly developing reduction of the amplitude of the lymphatic contractions, which increased
over time during continued flow. The inhibition of contraction amplitude was dependent on the direction of the imposed flow,
but independent of the magnitude of flow. Nitric oxide was partly but not completely responsible for the influence of flow
on the mesenteric lymph pump. Exposure to NO mimicked the effects of flow, and inhibition of the NO synthase by N G -monomethyl- l -arginine attenuated but did not completely abolish the effects of flow.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.2001.016642</identifier><identifier>PMID: 11986387</identifier><language>eng</language><publisher>Legacy CDMS: The Physiological Society</publisher><subject>Animals ; Blood Pressure - physiology ; In Vitro Techniques ; Kinetics ; Life Sciences (General) ; Lymph - physiology ; Lymph Nodes - physiology ; Lymphatic System - physiology ; Male ; Muscle Contraction - physiology ; Muscle, Smooth - physiology ; Pressure ; Rats ; Rats, Sprague-Dawley ; Research Papers ; Space life sciences ; Thoracic Duct - physiology</subject><ispartof>The Journal of physiology, 2002-05, Vol.540 (3), p.1023-1037</ispartof><rights>2002 The Journal of Physiology © 2002 The Physiological Society</rights><rights>The Physiological Society 2002 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4893-f926636252c144d3d193a82f701e8d712d8b5a17c120e554717e46e20dfa78ab3</citedby><cites>FETCH-LOGICAL-c4893-f926636252c144d3d193a82f701e8d712d8b5a17c120e554717e46e20dfa78ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290276/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290276/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11986387$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gashev, Anatoliy A.</creatorcontrib><creatorcontrib>Davis, Michael J.</creatorcontrib><creatorcontrib>Zawieja, David C.</creatorcontrib><creatorcontrib>Delp, M. D.</creatorcontrib><title>Inhibition of the active lymph pump by flow in rat mesenteric lymphatics and thoracic duct</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>There are only a few reports of the influence of imposed flow on an active lymph pump under conditions of controlled intraluminal
pressure. Thus, the mechanisms are not clearly defined. Rat mesenteric lymphatics and thoracic ducts were isolated, cannulated
and pressurized. Input and output pressures were adjusted to impose various flows. Lymphatic systolic and diastolic diameters
were measured and used to determine contraction frequency and pump flow indices. Imposed flow inhibited the active lymph pump
in both mesenteric lymphatics and in the thoracic duct. The active pump of the thoracic duct appeared more sensitive to flow
than did the active pump of the mesenteric lymphatics. Imposed flow reduced the frequency and amplitude of the contractions
and accordingly the active pump flow. Flow-induced inhibition of the active lymph pump followed two temporal patterns. The
first pattern was a rapidly developing inhibition of contraction frequency. Upon imposition of flow, the contraction frequency
immediately fell and then partially recovered over time during continued flow. This effect was dependent on the magnitude
of imposed flow, but did not depend on the direction of flow. The effect also depended upon the rate of change in the direction
of flow. The second pattern was a slowly developing reduction of the amplitude of the lymphatic contractions, which increased
over time during continued flow. The inhibition of contraction amplitude was dependent on the direction of the imposed flow,
but independent of the magnitude of flow. Nitric oxide was partly but not completely responsible for the influence of flow
on the mesenteric lymph pump. Exposure to NO mimicked the effects of flow, and inhibition of the NO synthase by N G -monomethyl- l -arginine attenuated but did not completely abolish the effects of flow.</description><subject>Animals</subject><subject>Blood Pressure - physiology</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Life Sciences (General)</subject><subject>Lymph - physiology</subject><subject>Lymph Nodes - physiology</subject><subject>Lymphatic System - physiology</subject><subject>Male</subject><subject>Muscle Contraction - physiology</subject><subject>Muscle, Smooth - physiology</subject><subject>Pressure</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Research Papers</subject><subject>Space life sciences</subject><subject>Thoracic Duct - physiology</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqNkc1u1DAUhSMEokPhDSrkFWKTwdd2_LNBQlWBokqwKBs2luM4jaskDnbSUd4eDxn-dqws-X7n87VOUVwA3gMAfXM_dWvyod8TjGGPgXNGHhU7YFyVQij6uNhhTEhJRQVnxbOU7jNHsVJPizMAJTmVYld8ux47X_vZhxGFFs2dQ8bO_sGhfh2mDk3LMKF6RW0fDsiPKJoZDS65cXbR2w0ys7cJmbHJ8RCNzffNYufnxZPW9Mm9OJ3nxdf3V7eXH8ubzx-uL9_dlJZJRctWEc4pJxWxwFhDG1DUSNIKDE42Akgj68qAsECwqyomQDjGHcFNa4Q0NT0v3m7eaakH19i8WzS9nqIfTFx1MF7_Oxl9p-_CgyZEYSJ4Frw6CWL4vrg068En6_rejC4sSQvgFeESZ5BtoI0hpeja348A1sdS9K9S9LEUvZWSYy__XvBP6NRCBtQGHHzv1v-S6ttPXwATmrMXW3Y0yej8wXTEGMZS0orl8ett3Pm77uCj05ssBevdvOqKYU31T9MPssq0Yw</recordid><startdate>20020501</startdate><enddate>20020501</enddate><creator>Gashev, Anatoliy A.</creator><creator>Davis, Michael J.</creator><creator>Zawieja, David C.</creator><creator>Delp, M. D.</creator><general>The Physiological Society</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science Inc</general><scope>CYE</scope><scope>CYI</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20020501</creationdate><title>Inhibition of the active lymph pump by flow in rat mesenteric lymphatics and thoracic duct</title><author>Gashev, Anatoliy A. ; Davis, Michael J. ; Zawieja, David C. ; Delp, M. D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4893-f926636252c144d3d193a82f701e8d712d8b5a17c120e554717e46e20dfa78ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Blood Pressure - physiology</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Life Sciences (General)</topic><topic>Lymph - physiology</topic><topic>Lymph Nodes - physiology</topic><topic>Lymphatic System - physiology</topic><topic>Male</topic><topic>Muscle Contraction - physiology</topic><topic>Muscle, Smooth - physiology</topic><topic>Pressure</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Research Papers</topic><topic>Space life sciences</topic><topic>Thoracic Duct - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gashev, Anatoliy A.</creatorcontrib><creatorcontrib>Davis, Michael J.</creatorcontrib><creatorcontrib>Zawieja, David C.</creatorcontrib><creatorcontrib>Delp, M. D.</creatorcontrib><collection>NASA Scientific and Technical Information</collection><collection>NASA Technical Reports Server</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gashev, Anatoliy A.</au><au>Davis, Michael J.</au><au>Zawieja, David C.</au><au>Delp, M. D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of the active lymph pump by flow in rat mesenteric lymphatics and thoracic duct</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>2002-05-01</date><risdate>2002</risdate><volume>540</volume><issue>3</issue><spage>1023</spage><epage>1037</epage><pages>1023-1037</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>There are only a few reports of the influence of imposed flow on an active lymph pump under conditions of controlled intraluminal
pressure. Thus, the mechanisms are not clearly defined. Rat mesenteric lymphatics and thoracic ducts were isolated, cannulated
and pressurized. Input and output pressures were adjusted to impose various flows. Lymphatic systolic and diastolic diameters
were measured and used to determine contraction frequency and pump flow indices. Imposed flow inhibited the active lymph pump
in both mesenteric lymphatics and in the thoracic duct. The active pump of the thoracic duct appeared more sensitive to flow
than did the active pump of the mesenteric lymphatics. Imposed flow reduced the frequency and amplitude of the contractions
and accordingly the active pump flow. Flow-induced inhibition of the active lymph pump followed two temporal patterns. The
first pattern was a rapidly developing inhibition of contraction frequency. Upon imposition of flow, the contraction frequency
immediately fell and then partially recovered over time during continued flow. This effect was dependent on the magnitude
of imposed flow, but did not depend on the direction of flow. The effect also depended upon the rate of change in the direction
of flow. The second pattern was a slowly developing reduction of the amplitude of the lymphatic contractions, which increased
over time during continued flow. The inhibition of contraction amplitude was dependent on the direction of the imposed flow,
but independent of the magnitude of flow. Nitric oxide was partly but not completely responsible for the influence of flow
on the mesenteric lymph pump. Exposure to NO mimicked the effects of flow, and inhibition of the NO synthase by N G -monomethyl- l -arginine attenuated but did not completely abolish the effects of flow.</abstract><cop>Legacy CDMS</cop><pub>The Physiological Society</pub><pmid>11986387</pmid><doi>10.1113/jphysiol.2001.016642</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Blood Pressure - physiology In Vitro Techniques Kinetics Life Sciences (General) Lymph - physiology Lymph Nodes - physiology Lymphatic System - physiology Male Muscle Contraction - physiology Muscle, Smooth - physiology Pressure Rats Rats, Sprague-Dawley Research Papers Space life sciences Thoracic Duct - physiology |
title | Inhibition of the active lymph pump by flow in rat mesenteric lymphatics and thoracic duct |
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