Transparent adult zebrafish as a tool for in vivo transplantation analysis

The zebrafish is a useful model for understanding normal and cancer stem cells, but analysis has been limited to embryogenesis due to the opacity of the adult fish. To address this, we have created a transparent adult zebrafish in which we transplanted either hematopoietic stem/progenitor cells or t...

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Bibliographic Details
Published in:Cell stem cell 2008-02, Vol.2 (2), p.183-189
Main Authors: White, Richard Mark, Sessa, Anna, Burke, Christopher, Bowman, Teresa, LeBlanc, Jocelyn, Ceol, Craig, Bourque, Caitlin, Dovey, Michael, Goessling, Wolfram, Burns, Caroline Erter, Zon, Leonard I
Format: Article
Language:English
Subjects:
Albinism - genetics
Animals
Danio rerio
Flow Cytometry
Light
Models, Animal
Neoplasm Transplantation
Pigmentation - genetics
Stem Cell Transplantation
Stem Cells - ultrastructure
Zebrafish
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Summary:The zebrafish is a useful model for understanding normal and cancer stem cells, but analysis has been limited to embryogenesis due to the opacity of the adult fish. To address this, we have created a transparent adult zebrafish in which we transplanted either hematopoietic stem/progenitor cells or tumor cells. In a hematopoiesis radiation recovery assay, transplantation of GFP-labeled marrow cells allowed for striking in vivo visual assessment of engraftment from 2 hr-5 weeks posttransplant. Using FACS analysis, both transparent and wild-type fish had equal engraftment, but this could only be visualized in the transparent recipient. In a tumor engraftment model, transplantation of RAS-melanoma cells allowed for visualization of tumor engraftment, proliferation, and distant metastases in as little as 5 days, which is not seen in wild-type recipients until 3 to 4 weeks. This transparent adult zebrafish serves as the ideal combination of both sensitivity and resolution for in vivo stem cell analyses.
ISSN:1934-5909
1875-9777
DOI:10.1016/j.stem.2007.11.002