Human immunodeficiency virus (HIV) RNA quantitation using the Amplicor HIV-1 Monitor test: not all yellow-top tubes are suitable for blood collection

Quantitative (HIV-1) RNA assays have gained increasing attention in the past year because of their usefulness in predicting disease progression and treatment efficacy (1, 2). These assays are now widely used for measuring HIV-1 load in plasma, and several HIV-1 RNA load tests are now commercially av...

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Bibliographic Details
Published in:Journal of clinical microbiology 1997-04, Vol.35 (4), p.1040-1040
Main Authors: Griffith, B P, Garner, R B, Gordon, M M
Format: Article
Language:English
Subjects:
AIDS/HIV
Blood Specimen Collection - standards
HIV Infections - virology
HIV-1 - genetics
human immunodeficiency virus 1
Humans
Polymerase Chain Reaction - methods
RNA, Viral - blood
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Summary:Quantitative (HIV-1) RNA assays have gained increasing attention in the past year because of their usefulness in predicting disease progression and treatment efficacy (1, 2). These assays are now widely used for measuring HIV-1 load in plasma, and several HIV-1 RNA load tests are now commercially available (3). Each of these assays has specific requirements regarding plasma preparation and the anticoagulant used in the blood collection tube. For example, acid-citrate-dextrose (ACD) (yellow-top) or EDTA (purple-top) tubes are recommended for use with the Amplicor HIV-1 Monitor test (Roche Diagnostics Systems). However, the inadvertant use of another type of yellow-top tube may result in invalid HIV RNA results because of PCR inhibition.
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.35.4.1040-1040.1997