Analysis of the role of the Spitzenkorper in fungal morphogenesis by computer simulation of apical branching in Aspergillus niger

• Published in: Proceedings of the National Academy of Sciences - PNAS 1997-08, Vol.94 (17), p.9096-9101
• Main Authors: Reynaga-Pena, C.G, Gierz, G, Bartnicki-Garcia, S
• Format: Article
• Language: English
• Subjects: APLICACIONES DEL ORDENADOR; APPLICATION DES ORDINATEURS; ASPERGILLUS NIGER; Aspergillus niger - growth & development; Bacteria; Biological Sciences; Biology; BRANCHING; Cell growth; COMPUTER APPLICATIONS; Computer based modeling; Computer Simulation; COMPUTER SOFTWARE; Condensation; CRECIMIENTO; CROISSANCE; CYTOPLASMIC ORGANELLES; Fungi; FUNGUS SIMULATOR PROGRAM; GROWTH; HYPHAE; HYPHAE ELONGATION; HYPHAL TIPS; IMAGENES; IMAGERIE; IMAGERY; LOGICIEL; MICELIO; Microscopy; Modeling; Models, Biological; MORFOGENESIS; MORPHOGENESE; MORPHOGENESIS; MUTANT; MUTANTES; MUTANTS; MYCELIUM; ORGANITE CELLULAIRE; ORGANULOS CITOPLASMICOS; PROGRAMAS DE ORDENADOR; RAMIFICACION; RAMIFICATION; Scientific imaging; SIMULACION; SIMULATION; Simulations; SUPPLY CENTER; Temperature; Trajectories; VESICLE
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.94.17.9096

High-resolution video microscopy, image analysis, and computer simulation were used to study the role of the Spitzenkorper (Spk) in apical branching of rhamnose-1, a temperature-sensitive mutant of Aspergillus niger. A shift to the restrictive temperature led to a cytoplasmic contraction that destabilized the Spk, causing its disappearance. After a short transition period, new Spk appeared where the two incipient apical branches emerged. Changes in cell shape, growth rate, and Spk position were recorded and transferred to the FUNGUS SIMULATOR program to test the hypothesis that the Spk functions as a vesicle supply center (VSC). The simulation faithfully duplicated the elongation of the main hypha and the two apical branches. Elongating hyphae exhibited the growth pattern described by the hyphoid equation. During the transition phase, when no Spk was visible, the growth pattern was nonhyphoid, with consecutive periods of isometric and asymmetric expansion; the apex became enlarged and blunt before the apical branches emerged. Video microscopy images suggested that the branch Spk were formed anew by gradual condensation of vesicle clouds. Simulation exercises where the VSC was split into two new VSCs failed to produce realistic shapes, thus supporting the notion that the branch Spk did not originate by division of the original Spk. The best computer simulation of apical branching morphogenesis included simulations of the ontogeny of branch Spk via condensation of vesicle clouds. This study supports the hypothesis that the Spk plays a major role in hyphal morphogenesis by operating as a VSC--i.e., by regulating the traffic of wall-building vesicles in the manner predicted by the hyphoid model