Interleukin‐15 up‐regulates the expression of CD154 on synovial fluid T cells

To investigate the role of the CD40-CD154 interaction in rheumatoid arthritis (RA), we analysed the expression of CD154 on CD3+ and CD4+ T cells in synovial fluid (SF) from patients with RA and in peripheral blood (PB) from patients and normal controls. As interleukin (IL)-15 is a potent activator o...

Full description

Saved in:
Bibliographic Details
Published in:Immunology 2000-06, Vol.100 (2), p.238-244
Main Authors: Möttönen, M., Isomäki, P., Luukkainen, R., Toivanen, P., Punnonen, J., Lassila, O.
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Arthritis, Rheumatoid - immunology
CD154 antigen
CD40 Ligand
Cell Culture Techniques
Humans
Immunologic Memory - immunology
Interleukin-15 - immunology
Lymphocyte Activation - immunology
Membrane Glycoproteins - metabolism
Middle Aged
Original
Palatine Tonsil - immunology
Synovial Fluid - immunology
T-Lymphocyte Subsets - immunology
Up-Regulation - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To investigate the role of the CD40-CD154 interaction in rheumatoid arthritis (RA), we analysed the expression of CD154 on CD3+ and CD4+ T cells in synovial fluid (SF) from patients with RA and in peripheral blood (PB) from patients and normal controls. As interleukin (IL)-15 is a potent activator of synovial T cells we wanted to study whether IL-15 also regulated the expression of CD154 on these T cells. Freshly isolated synovial T cells did not express significant levels of CD154, as evaluated using flow cytometry, whereas the expression of CD86 and human leucocyte antigen (HLA)-DR was significantly elevated on SF T cells when compared with PB T cells from patients or controls. Synovial T cells could up-regulate their CD154 expression following activation with phorbol 12-myristate 13-acetate (PMA) + ionomycin or anti-CD3 + anti-CD28 monoclonal antibodies (mAbs), but the maximal level of expression remained lower than in control T cells. IL-15 significantly increased the expression of CD154 on SF and PB T cells from patients, whereas IL-2 had minimal effects. Furthermore, IL-15 induced extensive proliferation in SF T cells. Our results show that SF T cells up-regulate the expression of CD154 in the presence of IL-15, a cytokine present in the synovium of patients with RA. These results further emphasize the role of IL-15 in the pathogenesis of RA.
ISSN:0019-2805
1365-2567
DOI:10.1046/j.1365-2567.2000.00023.x