Crystallization and preliminary X-ray diffraction analysis of maize aldose reductase

Maize aldose reductase (AR) is a member of the aldo‐keto reductase superfamily. In contrast to human AR, maize AR seems to prefer the conversion of sorbitol into glucose. The apoenzyme was crystallized in space group P212121, with unit‐cell parameters a = 47.2, b = 54.5, c = 100.6 Å and one molecule...

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Published in:Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2007-11, Vol.63 (11), p.990-992
Main Authors: Kiyota, Eduardo, De Sousa, Sylvia Morais, Dos Santos, Marcelo Leite, Da Costa Lima, Aline, Menossi, Marcelo, Yunes, José Andrés, Aparicio, Ricardo
Format: Article
Language:English
Subjects:
Aldehyde Reductase - chemistry
CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY
CONVERSION
CRYSTALLIZATION
Crystallization Communications
Crystallography, X-Ray
GLUCOSE
maize aldose reductase
MOLECULES
REDUCTION
RESOLUTION
SPACE GROUPS
SYNCHROTRONS
VISIBLE RADIATION
X-RAY DIFFRACTION
Zea mays - enzymology
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Summary:Maize aldose reductase (AR) is a member of the aldo‐keto reductase superfamily. In contrast to human AR, maize AR seems to prefer the conversion of sorbitol into glucose. The apoenzyme was crystallized in space group P212121, with unit‐cell parameters a = 47.2, b = 54.5, c = 100.6 Å and one molecule in the asymmetric unit. Synchrotron X‐ray diffraction data were collected and a final resolution limit of 2.0 Å was obtained after data reduction. Phasing was carried out by an automated molecular‐replacement procedure and structural refinement is currently in progress. The refined structure is expected to shed light on the functional/enzymatic mechanism and the unusual activities of maize AR.
ISSN:1744-3091
1744-3091
DOI:10.1107/S1744309107052670