Monoclonal antibody A7-superparamagnetic iron oxide as contrast agent of MR imaging of rectal carcinoma

Superparamagnetic iron oxide (SPIO)-based colloid has been used clinically as a tissue-specific magnetic resonance contrast agent. We coupled monoclonal antibody A7 (Mab A7), which reacts specifically with human colorectal carcinoma, to Ferumoxides (SPIO) and examined the accumulation of this conjug...

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Bibliographic Details
Published in:British journal of cancer 2005-07, Vol.93 (1), p.131-136
Main Authors: Toma, A, Otsuji, E, Kuriu, Y, Okamoto, K, Ichikawa, D, Hagiwara, A, Ito, H, Nishimura, T, Yamagishi, H
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - administration & dosage
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cancer Research
Colorectal Neoplasms - diagnosis
Colorectal Neoplasms - pathology
Contrast agents
Contrast Media
Drug Resistance
Epidemiology
Ferric Compounds - administration & dosage
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Magnetic Resonance Imaging
Medical prognosis
Medical research
Medical sciences
Mice
Mice, Inbred BALB C
Mice, Nude
Molecular Diagnostics
Molecular Medicine
Monoclonal antibodies
Neoplasm Recurrence, Local - diagnosis
Neoplasm Transplantation
Oncology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Surgery
Tumors
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Description
Summary:Superparamagnetic iron oxide (SPIO)-based colloid has been used clinically as a tissue-specific magnetic resonance contrast agent. We coupled monoclonal antibody A7 (Mab A7), which reacts specifically with human colorectal carcinoma, to Ferumoxides (SPIO) and examined the accumulation of this conjugate in xenografted tumours in nude mice. We examined in vitro immunoreactivity of Mab A7 coupled to Ferumoxides and its in vivo distribution in nude mice with human colorectal carcinoma. Magnetic resonance imaging of tumour-bearing nude mice was performed 72 h after injection of A7-Ferumoxides. A7-Ferumoxides retained binding activities that were nearly identical to intact Mab A7. More of the radiolabelled A7-Ferumoxides accumulated in the tumour than normal mouse IgG-Ferumoxides from 12 h onwards after injection ( P
ISSN:0007-0920
1532-1827
DOI:10.1038/sj.bjc.6602668