Monoclonal antibody ONS-M21 recognizes integrin α3 in gliomas and medulloblastomas

Summary The monoclonal antibody ONS-M21 recognizes an antigen found on the surface of glioma and medulloblastoma cells but does not react with the antigens of normal brain tissue. We purified and identified the 140-kDa protein by means of an antibody-binding affinity column. This 140-kDa antigen has...

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Bibliographic Details
Published in:British journal of cancer 1999-01, Vol.79 (2), p.333-339
Main Authors: Kishima, H, Shimizu, K, Tamura, K, Miyao, Y, Mabuchi, E, Tominaga, E, Matsuzaki, J, Hayakawa, T
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cancer Research
Drug Resistance
Epidemiology
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Miscellaneous. Technology
Molecular Medicine
Oncology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
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Summary:Summary The monoclonal antibody ONS-M21 recognizes an antigen found on the surface of glioma and medulloblastoma cells but does not react with the antigens of normal brain tissue. We purified and identified the 140-kDa protein by means of an antibody-binding affinity column. This 140-kDa antigen has sequences homologous to the amino-terminal region and five parts of the internal domain of integrin α3. When the integrin α3-related sequences was amplified and used to analyse the mRNA of glioma and medulloblastoma surgical specimens, the transcription level of integrin α3 mRNA appeared to be quantitatively correlated with the grade of malignancy. These findings suggest that the ONS-M21 antibody, which reacts with integrin α3, might be useful in the diagnosis of gliomas and medulloblastomas.
ISSN:0007-0920
1532-1827
DOI:10.1038/sj.bjc.6690053