Induction of reactive oxygen intermediates in human monocytes by tumour cells and their role in spontaneous monocyte cytotoxicity

• Published in: British journal of cancer 1999-02, Vol.79 (5-6), p.737-743
• Main Authors: Mytar, B, Siedlar, M, Woloszyn, M, Ruggiero, I, Pryjma, J, Zembala, M
• Format: Article
• Language: English
• Subjects: Adenocarcinoma; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Cancer Research; Carcinoma, Hepatocellular; Cell Division; Cell Line; Cell Survival; Drug Resistance; Epidemiology; Female; Flow Cytometry; Host-tumor relations. Immunology. Biological markers; Humans; Liver Neoplasms; Luminescent Measurements; Medical sciences; Molecular Medicine; Monocytes - cytology; Monocytes - drug effects; Monocytes - physiology; Oncology; Ovarian Neoplasms; Pancreatic Neoplasms; Reactive Oxygen Species - physiology; Regular; regular-article; Superoxides - blood; Tumor Cells, Cultured; Tumors
• ISSN: 0007-0920; 1532-1827
• DOI: 10.1038/sj.bjc.6690118

Summary The present study examined the ability of human monocytes to produce reactive oxygen intermediates after a contact with tumour cells. Monocytes generated oxygen radicals, as measured by luminol-enhanced chemiluminescence and superoxide anion production, after stimulation with the tumour, but not with untransformed, cells. The use of specific oxygen radical scavengers and inhibitors, superoxide dismutase, catalase, dimethyl sulphoxide and deferoxamine as well as the myeloperoxidase inhibitor 4-aminobenzoic acid hydrazide, indicated that chemiluminescence was dependent on the production of superoxide anion and hydroxyl radical and the presence of myeloperoxidase. The tumour cell-induced chemiluminescent response of monocytes showed different kinetics from that seen after activation of monocytes with phorbol ester. These results indicate that human monocytes can be directly stimulated by tumour cells for reactive oxygen intermediate production. Spontaneous monocyte-mediated cytotoxicity towards cancer cells was inhibited by superoxide dismutase, catalase, deferoxamine and hydrazide, implicating the role of superoxide anion, hydrogen peroxide, hydroxyl radical and hypohalite. We wish to suggest that so-called ‘spontaneous’ tumoricidal capacity of freshly isolated human monocytes may in fact be an inducible event associated with generation of reactive oxygen intermediates and perhaps other toxic mediators, resulting from a contact of monocytes with tumour cells.