Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy

Previous studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of thyroglobulin (TG) mRNA in the peripheral blood of patients with differentiated thyroid carcinoma. To evaluate this usefulness, we measured TG mRNA in the peripheral blood...

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Bibliographic Details
Published in:British journal of cancer 2001-07, Vol.85 (1), p.102-106
Main Authors: Takano, T, Miyauchi, A, Yoshida, H, Hasegawa, Y, Kuma, K, Amino, N
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cancer Research
Carcinoma
Dehydrogenases
Drug Resistance
Endocrinopathies
Epidemiology
Female
Glyceraldehyde-3-phosphate dehydrogenase
Glyceraldehyde-3-Phosphate Dehydrogenases - genetics
Humans
Male
Malignant tumors
Medical research
Medical sciences
Metastases
Metastasis
Middle Aged
Molecular Medicine
Oncology
Peripheral blood
Polymerase chain reaction
Regular
regular-article
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - blood
Statistics
Therapeutic applications
Thyroglobulin
Thyroglobulin - blood
Thyroglobulin - genetics
Thyroid cancer
thyroid carcinoma
Thyroid Neoplasms - blood
Thyroid Neoplasms - genetics
Thyroid Neoplasms - surgery
Thyroid. Thyroid axis (diseases)
Thyroidectomy
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Summary:Previous studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of thyroglobulin (TG) mRNA in the peripheral blood of patients with differentiated thyroid carcinoma. To evaluate this usefulness, we measured TG mRNA in the peripheral blood of patients diagnosed with thyroid carcinoma after total thyroidectomy by real-time quantitative RT-PCR using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA as an internal control. Surprisingly, we detected TG mRNA in all samples obtained after total thyroidectomy, including those from 4 medullary carcinomas. Further, there was no statistical difference in expression levels of TG mRNA in the patients with or without metastasis, and no significant correlation was found between serum TG concentrations and the expression levels of TG mRNA. These results give rise to a question regarding the clinical applications of not only RT-PCR detection but also quantitative measurement of TG mRNA in peripheral blood. © 2001 Cancer Research Campaign http://www.bjcancer.com
ISSN:0007-0920
1532-1827
DOI:10.1054/bjoc.2001.1904