Polo-like kinase is required for Golgi and bilobe biogenesis in Trypanosoma brucei

A bilobed structure marked by TbCentrin2 regulates Golgi duplication in the protozoan parasite Trypanosoma brucei. This structure must itself duplicate during the cell cycle for Golgi inheritance to proceed normally. We show here that duplication of the bilobed structure is dependent on the single p...

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Bibliographic Details
Published in:The Journal of cell biology 2008-05, Vol.181 (3), p.431-438
Main Authors: de Graffenried, Christopher L, Ho, Helen H, Warren, Graham
Format: Article
Language:English
Subjects:
Animals
Antibodies
Biochemistry
Calcium-Binding Proteins - genetics
Calcium-Binding Proteins - metabolism
Cell cycle
Cell Cycle - physiology
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell lines
Cellular biology
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - metabolism
Cytokinesis
Daughter cells
Endoplasmic Reticulum - metabolism
Endoplasmic Reticulum - ultrastructure
Eukaryotic cells
Flagella
Golgi Apparatus - metabolism
Golgi Apparatus - ultrastructure
HeLa cells
Kidney cells
Kinases
Organelles
Organelles - metabolism
Organelles - ultrastructure
Parasites
Parasitic protozoa
Polo-Like Kinase 1
Protein Isoforms - genetics
Protein Isoforms - metabolism
Protein Serine-Threonine Kinases - genetics
Protein Serine-Threonine Kinases - metabolism
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins - metabolism
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Trypanosoma brucei
Trypanosoma brucei brucei - cytology
Trypanosoma brucei brucei - metabolism
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Summary:A bilobed structure marked by TbCentrin2 regulates Golgi duplication in the protozoan parasite Trypanosoma brucei. This structure must itself duplicate during the cell cycle for Golgi inheritance to proceed normally. We show here that duplication of the bilobed structure is dependent on the single polo-like kinase (PLK) homologue in T. brucei (TbPLK). Depletion of TbPLK leads to malformed bilobed structures, which is consistent with an inhibition of duplication and an increase in the number of dispersed Golgi structures with associated endoplasmic reticulum exit sites. These data suggest that the bilobe may act as a scaffold for the controlled assembly of the duplicating Golgi.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200708082