Characterisation of human kallikrein 6 protease M expression in ovarian cancer

Kallikrein 6 (hK6, also known as protease M/zyme/neurosin) is a member of the human kallikrein gene family. We have previously cloned the cDNA for this gene by differential display and shown the overexpression of the mRNA in breast and ovarian primary tumour tissues and cell lines. To thoroughly cha...

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Bibliographic Details
Published in:British journal of cancer 2004-08, Vol.91 (4), p.725-731
Main Authors: Ni, X, Zhang, W, Huang, K-C, Wang, Y, Ng, S-K, Mok, S C, Berkowitz, R S, Ng, S-W
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Blotting, Southern
Cancer Research
Diagnosis, Differential
DNA, Neoplasm - analysis
Drug Resistance
Enzyme-Linked Immunosorbent Assay
Epidemiology
Female
Female genital diseases
Gene Amplification
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Genetic Markers
Gynecology. Andrology. Obstetrics
Humans
Immunohistochemistry
Kallikreins - biosynthesis
Medical sciences
Molecular and Cellular Pathology
Molecular Medicine
Neoplasm Staging
Oncology
Ovarian cancer
Ovarian Diseases - diagnosis
Ovarian Neoplasms - genetics
Ovarian Neoplasms - pathology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
Tumor Cells, Cultured
Tumors
Up-Regulation
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Summary:Kallikrein 6 (hK6, also known as protease M/zyme/neurosin) is a member of the human kallikrein gene family. We have previously cloned the cDNA for this gene by differential display and shown the overexpression of the mRNA in breast and ovarian primary tumour tissues and cell lines. To thoroughly characterise the expression of this kallikrein in ovarian cancer, we have developed a novel monoclonal antibody specific to hK6 and employed it in immunohistochemistry with a wide range of ovarian tumour samples. The expression was found elevated in 67 of 80 cases of ovarian tumour samples and there was a significant difference in the expression levels between normal and benign ovarian tissues and the borderline and invasive tumours ( P
ISSN:0007-0920
1532-1827
DOI:10.1038/sj.bjc.6602041