Transient expression of chicken alpha interferon gene in lettuce

We investigated the possibility of producing chicken alpha interferon （ChlFN-α） in transgenic plants. The cDNA encoding ChlFN-α was introduced into lettuce （Lactuca sativa L.） plants by using an agro-infiltration transient expression system. The ChlFN-a gene was correctly transcribed and translated...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2008-05, Vol.9 (5), p.351-355
Main Authors: Li, Song, De-gang, Zhao, Yong-jun, Wu, Yi, Li
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Chickens
Interferon Type I - biosynthesis
Interferon-alpha - genetics
Lactuca - genetics
Lactuca sativa
Lettuce
Plant Sciences
Plants, Genetically Modified - genetics
Recombinant Proteins
Science Letters
Vesicular stomatitis virus
基因表达
生物活性
鸡干扰素-α
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We investigated the possibility of producing chicken alpha interferon （ChlFN-α） in transgenic plants. The cDNA encoding ChlFN-α was introduced into lettuce （Lactuca sativa L.） plants by using an agro-infiltration transient expression system. The ChlFN-a gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays. Recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus （VSV） replication on chicken embryonic fibroblast （CEF）. The results demonstrate that biologically active avian cytokine with potential pharmaceutical applications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B0710596