Transient expression of chicken alpha interferon gene in lettuce

We investigated the possibility of producing chicken alpha interferon （ChlFN-α） in transgenic plants. The cDNA encoding ChlFN-α was introduced into lettuce （Lactuca sativa L.） plants by using an agro-infiltration transient expression system. The ChlFN-a gene was correctly transcribed and translated...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2008-05, Vol.9 (5), p.351-355
Main Authors: Li, Song, De-gang, Zhao, Yong-jun, Wu, Yi, Li
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Chickens
Interferon Type I - biosynthesis
Interferon-alpha - genetics
Lactuca - genetics
Lactuca sativa
Lettuce
Plant Sciences
Plants, Genetically Modified - genetics
Recombinant Proteins
Science Letters
Vesicular stomatitis virus
基因表达
生物活性
鸡干扰素-α
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c540t-32d7d35cd6835623a0b1dda670e883ee6ae39793da9c2454f99d8a3ae2e9b0ec3
cites cdi_FETCH-LOGICAL-c540t-32d7d35cd6835623a0b1dda670e883ee6ae39793da9c2454f99d8a3ae2e9b0ec3
container_end_page 355
container_issue 5
container_start_page 351
container_title Journal of Zhejiang University. B. Science
container_volume 9
creator Li, Song
De-gang, Zhao
Yong-jun, Wu
Yi, Li
description We investigated the possibility of producing chicken alpha interferon （ChlFN-α） in transgenic plants. The cDNA encoding ChlFN-α was introduced into lettuce （Lactuca sativa L.） plants by using an agro-infiltration transient expression system. The ChlFN-a gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays. Recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus （VSV） replication on chicken embryonic fibroblast （CEF）. The results demonstrate that biologically active avian cytokine with potential pharmaceutical applications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.
doi_str_mv 10.1631/jzus.B0710596
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2367372</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>27054802</cqvip_id><sourcerecordid>2425035961</sourcerecordid><originalsourceid>FETCH-LOGICAL-c540t-32d7d35cd6835623a0b1dda670e883ee6ae39793da9c2454f99d8a3ae2e9b0ec3</originalsourceid><addsrcrecordid>eNqFkUtv1DAUhS0Eog9YskURC3YZrn0TPzYIqApUqsSmrC2PczPjacaZ2gkCfj2uZqAUCXVlW-fzuY_D2AsOCy6Rv9n8nPPiAygOrZGP2DHXUtRcaXxc7lJhzVvNj9hJzhuApgEln7IjrlsApfCYvbtKLuZAcaro-y5RzmGM1dhXfh38NcXKDbu1q0KcKPWUiraiSOVdDTRNs6dn7EnvhkzPD-cp-_rx_Orsc3355dPF2fvL2rcNTDWKTnXY-k5qbKVAB0vedU4qIK2RSDpCowx2znjRtE1vTKcdOhJklkAeT9nbve9uXm6p86Xj5Aa7S2Hr0g87umDvKzGs7Wr8ZgWWLShRDF4fDNJ4M1Oe7DZkT8PgIo1ztgpUI3XLHwQRuRFSwIOgAOAGwRTw1T_gZpxTLOuyZXjUQoEsUL2HfBpzTtT_mY2DvY3a3kZtf0dd-Jd_L-SOPmRbgMUeyEWKK0p3Vf_neGjTr8e4uil_7NL56z4MZEuLbaNB4C-iwcBL</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>883382706</pqid></control><display><type>article</type><title>Transient expression of chicken alpha interferon gene in lettuce</title><source>Springer Link</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Li, Song ; De-gang, Zhao ; Yong-jun, Wu ; Yi, Li</creator><creatorcontrib>Li, Song ; De-gang, Zhao ; Yong-jun, Wu ; Yi, Li</creatorcontrib><description>We investigated the possibility of producing chicken alpha interferon （ChlFN-α） in transgenic plants. The cDNA encoding ChlFN-α was introduced into lettuce （Lactuca sativa L.） plants by using an agro-infiltration transient expression system. The ChlFN-a gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays. Recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus （VSV） replication on chicken embryonic fibroblast （CEF）. The results demonstrate that biologically active avian cytokine with potential pharmaceutical applications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.</description><identifier>ISSN: 1673-1581</identifier><identifier>EISSN: 1862-1783</identifier><identifier>DOI: 10.1631/jzus.B0710596</identifier><identifier>PMID: 18500773</identifier><language>eng</language><publisher>Heidelberg: SP Zhejiang University Press</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; Chickens ; Interferon Type I - biosynthesis ; Interferon-alpha - genetics ; Lactuca - genetics ; Lactuca sativa ; Lettuce ; Plant Sciences ; Plants, Genetically Modified - genetics ; Recombinant Proteins ; Science Letters ; Vesicular stomatitis virus ; 基因表达 ; 生物活性 ; 鸡干扰素-α</subject><ispartof>Journal of Zhejiang University. B. Science, 2008-05, Vol.9 (5), p.351-355</ispartof><rights>Zhejiang University 2008</rights><rights>Copyright © 2008, Journal of Zhejiang University Science 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-32d7d35cd6835623a0b1dda670e883ee6ae39793da9c2454f99d8a3ae2e9b0ec3</citedby><cites>FETCH-LOGICAL-c540t-32d7d35cd6835623a0b1dda670e883ee6ae39793da9c2454f99d8a3ae2e9b0ec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86281A/86281A.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2367372/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2367372/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18500773$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Song</creatorcontrib><creatorcontrib>De-gang, Zhao</creatorcontrib><creatorcontrib>Yong-jun, Wu</creatorcontrib><creatorcontrib>Yi, Li</creatorcontrib><title>Transient expression of chicken alpha interferon gene in lettuce</title><title>Journal of Zhejiang University. B. Science</title><addtitle>J. Zhejiang Univ. Sci. B</addtitle><addtitle>Journal of Zhejiang University Science</addtitle><description>We investigated the possibility of producing chicken alpha interferon （ChlFN-α） in transgenic plants. The cDNA encoding ChlFN-α was introduced into lettuce （Lactuca sativa L.） plants by using an agro-infiltration transient expression system. The ChlFN-a gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays. Recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus （VSV） replication on chicken embryonic fibroblast （CEF）. The results demonstrate that biologically active avian cytokine with potential pharmaceutical applications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Chickens</subject><subject>Interferon Type I - biosynthesis</subject><subject>Interferon-alpha - genetics</subject><subject>Lactuca - genetics</subject><subject>Lactuca sativa</subject><subject>Lettuce</subject><subject>Plant Sciences</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Recombinant Proteins</subject><subject>Science Letters</subject><subject>Vesicular stomatitis virus</subject><subject>基因表达</subject><subject>生物活性</subject><subject>鸡干扰素-α</subject><issn>1673-1581</issn><issn>1862-1783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkUtv1DAUhS0Eog9YskURC3YZrn0TPzYIqApUqsSmrC2PczPjacaZ2gkCfj2uZqAUCXVlW-fzuY_D2AsOCy6Rv9n8nPPiAygOrZGP2DHXUtRcaXxc7lJhzVvNj9hJzhuApgEln7IjrlsApfCYvbtKLuZAcaro-y5RzmGM1dhXfh38NcXKDbu1q0KcKPWUiraiSOVdDTRNs6dn7EnvhkzPD-cp-_rx_Orsc3355dPF2fvL2rcNTDWKTnXY-k5qbKVAB0vedU4qIK2RSDpCowx2znjRtE1vTKcdOhJklkAeT9nbve9uXm6p86Xj5Aa7S2Hr0g87umDvKzGs7Wr8ZgWWLShRDF4fDNJ4M1Oe7DZkT8PgIo1ztgpUI3XLHwQRuRFSwIOgAOAGwRTw1T_gZpxTLOuyZXjUQoEsUL2HfBpzTtT_mY2DvY3a3kZtf0dd-Jd_L-SOPmRbgMUeyEWKK0p3Vf_neGjTr8e4uil_7NL56z4MZEuLbaNB4C-iwcBL</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Li, Song</creator><creator>De-gang, Zhao</creator><creator>Yong-jun, Wu</creator><creator>Yi, Li</creator><general>SP Zhejiang University Press</general><general>Springer Nature B.V</general><general>Zhejiang University Press</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>RC3</scope><scope>7SC</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080501</creationdate><title>Transient expression of chicken alpha interferon gene in lettuce</title><author>Li, Song ; De-gang, Zhao ; Yong-jun, Wu ; Yi, Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-32d7d35cd6835623a0b1dda670e883ee6ae39793da9c2454f99d8a3ae2e9b0ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Chickens</topic><topic>Interferon Type I - biosynthesis</topic><topic>Interferon-alpha - genetics</topic><topic>Lactuca - genetics</topic><topic>Lactuca sativa</topic><topic>Lettuce</topic><topic>Plant Sciences</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Recombinant Proteins</topic><topic>Science Letters</topic><topic>Vesicular stomatitis virus</topic><topic>基因表达</topic><topic>生物活性</topic><topic>鸡干扰素-α</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Song</creatorcontrib><creatorcontrib>De-gang, Zhao</creatorcontrib><creatorcontrib>Yong-jun, Wu</creatorcontrib><creatorcontrib>Yi, Li</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric &amp; Aquatic Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric &amp; Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Genetics Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Electronics &amp; Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical &amp; Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts – Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Zhejiang University. B. Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Song</au><au>De-gang, Zhao</au><au>Yong-jun, Wu</au><au>Yi, Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transient expression of chicken alpha interferon gene in lettuce</atitle><jtitle>Journal of Zhejiang University. B. Science</jtitle><stitle>J. Zhejiang Univ. Sci. B</stitle><addtitle>Journal of Zhejiang University Science</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>9</volume><issue>5</issue><spage>351</spage><epage>355</epage><pages>351-355</pages><issn>1673-1581</issn><eissn>1862-1783</eissn><abstract>We investigated the possibility of producing chicken alpha interferon （ChlFN-α） in transgenic plants. The cDNA encoding ChlFN-α was introduced into lettuce （Lactuca sativa L.） plants by using an agro-infiltration transient expression system. The ChlFN-a gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays. Recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus （VSV） replication on chicken embryonic fibroblast （CEF）. The results demonstrate that biologically active avian cytokine with potential pharmaceutical applications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.</abstract><cop>Heidelberg</cop><pub>SP Zhejiang University Press</pub><pmid>18500773</pmid><doi>10.1631/jzus.B0710596</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1673-1581
ispartof Journal of Zhejiang University. B. Science, 2008-05, Vol.9 (5), p.351-355
issn 1673-1581
1862-1783
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2367372
source Springer Link; PubMed Central; Free Full-Text Journals in Chemistry
subjects Animals
Biomedical and Life Sciences
Biomedicine
Chickens
Interferon Type I - biosynthesis
Interferon-alpha - genetics
Lactuca - genetics
Lactuca sativa
Lettuce
Plant Sciences
Plants, Genetically Modified - genetics
Recombinant Proteins
Science Letters
Vesicular stomatitis virus
基因表达
生物活性
鸡干扰素-α
title Transient expression of chicken alpha interferon gene in lettuce
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T16%3A57%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transient%20expression%20of%20chicken%20alpha%20interferon%20gene%20in%20lettuce&rft.jtitle=Journal%20of%20Zhejiang%20University.%20B.%20Science&rft.au=Li,%20Song&rft.date=2008-05-01&rft.volume=9&rft.issue=5&rft.spage=351&rft.epage=355&rft.pages=351-355&rft.issn=1673-1581&rft.eissn=1862-1783&rft_id=info:doi/10.1631/jzus.B0710596&rft_dat=%3Cproquest_pubme%3E2425035961%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c540t-32d7d35cd6835623a0b1dda670e883ee6ae39793da9c2454f99d8a3ae2e9b0ec3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=883382706&rft_id=info:pmid/18500773&rft_cqvip_id=27054802&rfr_iscdi=true