Prussian Blue Dispersed Sphere Catalytic Labels for Amplified Electronic Detection of DNA

A highly sensitive electrochemical DNA hybridization assay using Prussian blue (PB)‐modified polymeric spheres as the oligonculeotide labeling tag is described. The sandwich assay relies on a secondary nucleic‐acid probe functionalized with polystyrene beads loaded with numerous Prussian blue nanopa...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2008-02, Vol.20 (3), p.308-312
Main Authors: Suwansa-ard, Siriwan, Xiang, Yun, Bash, Ralph, Thavarungkul, Panote, Kanatharana, Proespichaya, Wang, Joseph
Format: Article
Language:English
Subjects:
Amplification
DNA hybridization
Microspheres
Prussian blue
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Summary:A highly sensitive electrochemical DNA hybridization assay using Prussian blue (PB)‐modified polymeric spheres as the oligonculeotide labeling tag is described. The sandwich assay relies on a secondary nucleic‐acid probe functionalized with polystyrene beads loaded with numerous Prussian blue nanoparticles. The very strong catalytic activity of the captured PB ‘artificial peroxidase’ tag towards the reduction of hydrogen peroxide, along with the encapsulation of numerous catalytic particles onto polymeric beads, allows amperometric detection of the DNA target down to the 50 fM level (2.5 amol). Imaging and spectroscopic measurements are used to characterize the PB‐tagged polystyrene beads. Such coupling of PB catalytic labels with polymeric carrier beads offers great promise for amplified transduction of different biomolecular interactions.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.200704063