Identification of mRNAs differentially-expressed between benign and malignant breast tumour cells

Two suppression subtracted cDNA libraries have been constructed, one containing cDNAs to mRNAs present at a higher level in a benign human breast tumour-derived cell line relative to the malignant mammary cell line, MCF-7, and the other containing cDNAs present at a higher level in the MCF-7 cells r...

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Bibliographic Details
Published in:British journal of cancer 2002-08, Vol.87 (4), p.423-431
Main Authors: Liu, D, Rudland, P S, Sibson, D R, Barraclough, R
Format: Article
Language:English
Subjects:
Antigens
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Breast - metabolism
Breast cancer
Breast Neoplasms - metabolism
Cancer Research
Cell culture
Chromosomes, Human, Pair 21
Cloning
Drug Resistance
Epidemiology
Expressed Sequence Tags
Gene Expression
Gene Library
Gynecology. Andrology. Obstetrics
Humans
In Situ Hybridization
Insulin
Mammary gland diseases
Medical research
Medical sciences
Metastasis
Molecular and Cellular Pathology
Molecular Medicine
Oncology
RNA, Messenger - metabolism
Tumor Cells, Cultured
Tumors
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Summary:Two suppression subtracted cDNA libraries have been constructed, one containing cDNAs to mRNAs present at a higher level in a benign human breast tumour-derived cell line relative to the malignant mammary cell line, MCF-7, and the other containing cDNAs present at a higher level in the MCF-7 cells relative to the benign cells. Randomly-picked cloned DNAs have been sequenced yielding 29 and 128 different cDNAs from the benign and malignant libraries, respectively. Using reverse Northern hybridisation, 76% and 83% of the cDNAs were differentially expressed by greater than two-fold, whilst 14% and 11% of cDNAs in the respective libraries were differentially expressed by more than 15-fold. Amongst these were oestrogen-responsive cDNAs and expressed sequence tags. One such oestrogen-responsive expressed sequence tag, M41, is transcribed from a gene located on chromosome 21q22.3, within an intron of a larger gene. The M41 gene contains oestrogen response elements, one of which is associated with alu repeats. M41 mRNA is expressed at a statistically significantly higher level in human breast cancer specimens than in normal human breast and benign lesions. In carcinomas, its up-regulation is associated with the development of the malignant cell.
ISSN:0007-0920
1532-1827
DOI:10.1038/sj.bjc.6600456