Selective accumulation of ALA-induced PpIX and photodynamic effect in chemically induced hepatocellular carcinoma

The possibility of 5-aminolaevulinic acid-based photodynamic therapy (ALA-PDT) for liver cancer was investigated using a chemically induced hepatocellular carcinoma (HCC) model. Endogenously synthesised protoporphyrin IX (PpIX) following the administration of ALA is an effective photosensitiser for...

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Bibliographic Details
Published in:British journal of cancer 2003-08, Vol.89 (4), p.730-736
Main Authors: Otake, M, Nishiwaki, M, Kobayashi, Y, Baba, S, Kohno, E, Kawasaki, T, Fujise, Y, Nakamura, H
Format: Article
Language:English
Subjects:
Acids
Alkylating Agents - toxicity
Aminolevulinic Acid - therapeutic use
Animals
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Biosynthesis
Cancer Research
Carcinoma, Hepatocellular - chemically induced
Carcinoma, Hepatocellular - drug therapy
Carcinoma, Hepatocellular - metabolism
Cell Division
Diethylnitrosamine - toxicity
Drug Resistance
Epidemiology
Experimental Therapeutics
Lasers
Liver - drug effects
Liver - metabolism
Liver cancer
Liver Neoplasms, Experimental - chemically induced
Liver Neoplasms, Experimental - drug therapy
Liver Neoplasms, Experimental - metabolism
Male
Medical research
Medical sciences
Medicine
Molecular Medicine
Oncology
Photochemotherapy
Photodynamic therapy
Photoradiation therapy and photosensitizing agent
Photosensitizing Agents - metabolism
Photosensitizing Agents - therapeutic use
Protoporphyrins - metabolism
Rats
Rats, Inbred F344
Spectrometry, Fluorescence
Treatment with physical agents
Treatment. General aspects
Tumors
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Summary:The possibility of 5-aminolaevulinic acid-based photodynamic therapy (ALA-PDT) for liver cancer was investigated using a chemically induced hepatocellular carcinoma (HCC) model. Endogenously synthesised protoporphyrin IX (PpIX) following the administration of ALA is an effective photosensitiser for PDT. We determined the fluorescence intensity of PpIX in HCC and nontumoral tissue in the liver. 5-Aminolaevulinic acid was intravenously injected to male Fisher rats with HCC at a dose of 500 mg kg −1 , and the fluorescence intensity in each tissue sample excised from liver was measured with a spectrofluorometer at 1, 3 and 6 h after administration. Fluorescence intensity was at a peak of 3 h after administration in both HCC and nontumoral tissue. The accumulation of PpIX in HCC was higher than that in the nontumoral tissue at 1 h ( P
ISSN:0007-0920
1532-1827
DOI:10.1038/sj.bjc.6601135