Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane

In this paper, we report a new method of fabricating a high-throughput protein preconcentrator in poly(dimethylsiloxane) (PDMS) microfluidic chip format. We print a submicron thick ion-selective membrane on the glass substrate by using standard patterning techniques. By simply plasma-bonding a PDMS...

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Bibliographic Details
Published in:Lab on a chip 2008-01, Vol.8 (4), p.596-601
Main Authors: Lee, Jeong Hoon, Song, Yong-Ak, Han, Jongyoon
Format: Article
Language:English
Subjects:
Dimethylpolysiloxanes - chemistry
Glass - chemistry
Ion-Selective Electrodes
Mass Spectrometry - methods
Membranes, Artificial
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microscopy, Fluorescence - methods
Proteomics - instrumentation
Proteomics - methods
Surface Properties
Time Factors
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Summary:In this paper, we report a new method of fabricating a high-throughput protein preconcentrator in poly(dimethylsiloxane) (PDMS) microfluidic chip format. We print a submicron thick ion-selective membrane on the glass substrate by using standard patterning techniques. By simply plasma-bonding a PDMS microfluidic device on top of the printed glass substrate, we can integrate the ion-selective membrane into the device and rapidly prototype a PDMS preconcentrator without complicated microfabrication and cumbersome integration processes. The PDMS preconcentrator shows a concentration factor as high as approximately 10(4) in 5 min. This printing method even allows fabricating a parallel array of preconcentrators to increase the concentrated sample volume, which can facilitate an integration of our microfluidic preconcentrator chip as a signal enhancing tool to various detectors such as a mass spectrometer.
ISSN:1473-0197
1473-0189
DOI:10.1039/b717900f