Defining the Epithelial Stem Cell Niche in Skin

Many adult regenerative cells divide infrequently but have high proliferative capacity. We developed a strategy to fluorescently label slow-cycling cells in a cell type-specific fashion. We used this method to purify the label-retaining cells (LRCs) that mark the skin stem cell (SC) niche. We found...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2004-01, Vol.303 (5656), p.359-363
Main Authors: Tumbar, Tudorita, Guasch, Geraldine, Greco, Valentina, Blanpain, Cedric, Lowry, William E., Rendl, Michael, Fuchs, Elaine
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biological and medical sciences
Cell Cycle
Cell differentiation, maturation, development, hematopoiesis
Cell Division
Cell growth
Cell physiology
Cell Separation
Cells
Epidermal Cells
Epidermis - physiology
Epithelial cells
Epithelial Cells - cytology
Epithelial Cells - physiology
Fluorescence
Follicles
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Gene Expression Regulation
Green Fluorescent Proteins
Hair
Hair Follicle - cytology
Hair Follicle - physiology
Histones - genetics
Histones - metabolism
Individualized Instruction
Keratinocytes - cytology
Learning Resources Centers
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Messenger RNA
Mice
Mice, Transgenic
Microscopy, Fluorescence
Molecular and cellular biology
Multipotent Stem Cells - cytology
Multipotent Stem Cells - physiology
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Skin
Stem cells
Tissues
Transcription, Genetic
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Description
Summary:Many adult regenerative cells divide infrequently but have high proliferative capacity. We developed a strategy to fluorescently label slow-cycling cells in a cell type-specific fashion. We used this method to purify the label-retaining cells (LRCs) that mark the skin stem cell (SC) niche. We found that these cells rarely divide within their niche but change properties abruptly when stimulated to exit. We determined their transcriptional profile, which, when compared to progeny and other SCs, defines the niche. Many of the > 100 messenger RNAs preferentially expressed in the niche encode surface receptors and secreted proteins, enabling LRCs to signal and respond to their environment.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1092436