Docosahexaenoic Acid Enhances Segregation of Lipids between Raft and Nonraft Domains: 2H-NMR Study

Solid-state 2H-NMR of [2H31]-N-palmitoylsphingomyelin ([2H31]16:0SM, PSM*), supplemented by differential scanning calorimetry, was used for the first time, to our knowledge, to investigate the molecular organization of the sphingolipid in 1:1:1mol mixtures with 1-palmitoyl-2-oleoyl-sn-glycero-3-phos...

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Bibliographic Details
Published in:Biophysical journal 2008-07, Vol.95 (1), p.203-214
Main Authors: Soni, Smita P., LoCascio, Daniel S., Liu, Yidong, Williams, Justin A., Bittman, Robert, Stillwell, William, Wassall, Stephen R.
Format: Article
Language:English
Subjects:
Cholesterol
Differential scanning calorimetry
Docosahexaenoic acid
Lipids
Membranes
Molecular interactions
Rafts
Sterols
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Summary:Solid-state 2H-NMR of [2H31]-N-palmitoylsphingomyelin ([2H31]16:0SM, PSM*), supplemented by differential scanning calorimetry, was used for the first time, to our knowledge, to investigate the molecular organization of the sphingolipid in 1:1:1mol mixtures with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (16:0–18:1PE, POPE) or 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (16:0–22:6PE, PDPE) and cholesterol. When compared with 2H-NMR data for analogous mixtures of [2H31]16:0–18:1PE (POPE*) or [2H31]16:0-22:6PE (PDPE*) with egg SM and cholesterol, molecular interactions of oleic acid (OA) versus docosahexaenoic acid (DHA) are distinguished, and details of membrane architecture emerge. SM-rich, characterized by higher-order, and PE-rich, characterized by lower-order, domains
ISSN:0006-3495
1542-0086
DOI:10.1529/biophysj.107.123612