Small-Molecule CFTR Inhibitors Slow Cyst Growth in Polycystic Kidney Disease

Cyst expansion in polycystic kidney disease (PKD) involves progressive fluid accumulation, which is believed to require chloride transport by the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Herein is reported that small-molecule CFTR inhibitors of the thiazolidinone and glyci...

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Bibliographic Details
Published in:Journal of the American Society of Nephrology 2008-07, Vol.19 (7), p.1300-1310
Main Authors: BAOXUE YANG, SONAWANE, Nitin D, DAN ZHAO, SOMLO, Stefan, VERKMAN, A. S
Format: Article
Language:English
Subjects:
Animals
Basic Research
Biological and medical sciences
Cadherins - genetics
Cell Line
Cystic Fibrosis Transmembrane Conductance Regulator - antagonists & inhibitors
Dogs
Glycine - analogs & derivatives
Glycine - pharmacology
Glycine - therapeutic use
Integrases - genetics
Integrases - metabolism
Kidney - pathology
Kidneys
Malformations of the urinary system
Medical sciences
Mice
Mice, Inbred C57BL
Nephrology. Urinary tract diseases
Polycystic Kidney, Autosomal Dominant - drug therapy
Polycystic Kidney, Autosomal Dominant - pathology
Thiazolidines - pharmacology
Thiazolidines - therapeutic use
TRPP Cation Channels
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Summary:Cyst expansion in polycystic kidney disease (PKD) involves progressive fluid accumulation, which is believed to require chloride transport by the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Herein is reported that small-molecule CFTR inhibitors of the thiazolidinone and glycine hydrazide classes slow cyst expansion in in vitro and in vivo models of PKD. More than 30 CFTR inhibitor analogs were screened in an MDCK cell model, and near-complete suppression of cyst growth was found by tetrazolo-CFTR(inh)-172, a tetrazolo-derived thiazolidinone, and Ph-GlyH-101, a phenyl-derived glycine hydrazide, without an effect on cell proliferation. These compounds also inhibited cyst number and growth by >80% in an embryonic kidney cyst model involving 4-d organ culture of embryonic day 13.5 mouse kidneys in 8-Br-cAMP-containing medium. Subcutaneous delivery of tetrazolo-CFTR(inh)-172 and Ph-GlyH-101 to neonatal, kidney-specific PKD1 knockout mice produced stable, therapeutic inhibitor concentrations of >3 microM in urine and kidney tissue. Treatment of mice for up to 7 d remarkably slowed kidney enlargement and cyst expansion and preserved renal function. These results implicate CFTR in renal cyst growth and suggest that CFTR inhibitors may hold therapeutic potential to reduce cyst growth in PKD.
ISSN:1046-6673
1533-3450
DOI:10.1681/asn.2007070828