Mosquito densonucleosis virus non-structural protein NS2 is necessary for a productive infection

Abstract Mosquito densonucleosis viruses synthesize two non-structural proteins, NS1 and NS2. While NS1 has been studied relatively well, little is known about NS2. Antiserum was raised against a peptide near the N-terminus of NS2, and used to conduct Western blot analysis and immuno-fluorescence as...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2008-04, Vol.374 (1), p.128-137
Main Authors: Azarkh, Eugene, Robinson, Erin, Hirunkanokpun, Supanee, Afanasiev, Boris, Kittayapong, Pattamaporn, Carlson, Jonathan, Corsini, Joe
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Amino Acid Substitution - genetics
AMINO ACIDS
Animals
ANTIBODIES
Brevidensovirus
Cell Line
Cell Nucleus - chemistry
Culicidae
Culicidae - virology
Cytoplasm - chemistry
Densovirinae - genetics
Densovirinae - physiology
Densovirus
FLUORESCENCE
fluorescent antibody technique
Gene Deletion
IMMUNE SERUMS
Immuno-fluorescent assay
Infectious Disease
Microbial Viability - genetics
Microscopy, Fluorescence
Mosquito densonucleosis viruses
MOSQUITOES
MUTATIONS
Non-structural proteins
nuclear localization signal
Parvoviridae
pathogenesis
PEPTIDES
TIME DEPENDENCE
Time Factors
Viral Nonstructural Proteins - analysis
Viral Nonstructural Proteins - genetics
Viral Nonstructural Proteins - physiology
viral proteins
Virus Replication - genetics
VIRUSES
Western analysis
Western blotting
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Summary:Abstract Mosquito densonucleosis viruses synthesize two non-structural proteins, NS1 and NS2. While NS1 has been studied relatively well, little is known about NS2. Antiserum was raised against a peptide near the N-terminus of NS2, and used to conduct Western blot analysis and immuno-fluorescence assays. Western blots revealed a prominent band near the expected size (41 kDa). Immuno-fluorescence studies of mosquito cells transfected with Ae DNV indicate that NS2 has a wider distribution pattern than does NS1, and the distribution pattern appears to be a function of time post-infection. Nuclear localization of NS2 requires intact C-terminus but does not require additional viral proteins. Mutations ranging from complete NS2 knock-out to a single missense amino acid substitution in NS2 can significantly reduce viral replication and production of viable progeny.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2007.11.035