Establishing humanized mice using stem cells: maximizing the potential

Studies on physiology and pathology as they relate to the immune system draw heavily upon rodent models. With the increasing impetus provided by initiatives in translational medicine, the demand for ever more sophisticated, 'humanized' murine models is greater than ever. However, the desig...

Full description

Saved in:
Bibliographic Details
Published in:Clinical and experimental immunology 2008-06, Vol.152 (3), p.406-414
Main Authors: Bernard, D, Peakman, M, Hayday, A.C
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Antigens, CD34 - analysis
Biological and medical sciences
CD34
Cell Separation - methods
cord blood
Disease Models, Animal
Fundamental and applied biological sciences. Psychology
Hematopoietic Stem Cell Transplantation - methods
humanized mouse
Humans
Mice
Mice, SCID
Molecular biophysics
Review
Transplantation, Heterologous
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Studies on physiology and pathology as they relate to the immune system draw heavily upon rodent models. With the increasing impetus provided by initiatives in translational medicine, the demand for ever more sophisticated, 'humanized' murine models is greater than ever. However, the design and implementation of studies in such mice is far from trivial. Here we provide a technical perspective on the increasing interest in developing humanized mice. We give examples of primary data starting with the routine procurement of human donor material, through CD34⁺ cell purification prior to engraftment to injection into immunocompromised mice. Our goal is to provide practical advice to the many investigators who may be commencing or considering such studies.
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.2008.03659.x