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The problem of cultivation of Mycobacterium leprae. A review with criteria for evaluating recent experimental work
Some criteria are presented to help evaluate papers appearing in the literature claiming successful cultivation of M. leprae either in the absence or in the presence of tissue-cultured cells. Recently, electron microscopic studies have definitely shown M. leprae to belong to the genus Mycobacterium...
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| Published in: | Bulletin of the World Health Organization 1973, Vol.49 (4), p.403-410 |
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| Format: | Article |
| Language: | English |
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| container_end_page | 410 |
| container_issue | 4 |
| container_start_page | 403 |
| container_title | Bulletin of the World Health Organization |
| container_volume | 49 |
| creator | Pattyn, S R |
| description | Some criteria are presented to help evaluate papers appearing in the literature claiming successful cultivation of M. leprae either in the absence or in the presence of tissue-cultured cells. Recently, electron microscopic studies have definitely shown M. leprae to belong to the genus Mycobacterium and its division to occur through transverse section. A survey is given of the mycobacterial strains isolated in the last 10 years from leprosy lesions. These strains belong to taxonomically different species and cannot be considered to be M. leprae. No substantiated claim was made concerning the in vitro growth of M. leprae and the application of the tissue culture technique has been equally disappointing. The view is expressed that progress towards the in vitro cultivation of M. leprae can be made only as a result of increased knowledge about the intracellular environment and the metabolic activities of this organism, to be obtained by the application of modern biochemical and histochemical techniques. |
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The view is expressed that progress towards the in vitro cultivation of M. leprae can be made only as a result of increased knowledge about the intracellular environment and the metabolic activities of this organism, to be obtained by the application of modern biochemical and histochemical techniques.</description><identifier>ISSN: 0042-9686</identifier><identifier>PMID: 4212439</identifier><language>eng</language><publisher>Switzerland</publisher><subject><![CDATA[Animals ; Bacteriological Techniques ; Cell Division ; Cell Line ; Cells, Cultured ; Culture Media ; Culture Techniques ; Ganglia ; Haplorhini ; Humans ; L Forms - isolation & purification ; Leprosy - microbiology ; Macrophages ; Mice ; Microscopy, Electron ; Mycobacterium - classification ; Mycobacterium - growth & development ; Mycobacterium - isolation & purification ; Mycobacterium leprae - cytology ; Mycobacterium leprae - growth & development ; Mycobacterium leprae - isolation & purification ; Mycobacterium leprae - metabolism ; Mycobacterium lepraemurium - growth & development ; Mycobacterium lepraemurium - isolation & purification ; Mycobacterium lepraemurium - metabolism ; Rats]]></subject><ispartof>Bulletin of the World Health Organization, 1973, Vol.49 (4), p.403-410</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2480944/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2480944/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4212439$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pattyn, S R</creatorcontrib><title>The problem of cultivation of Mycobacterium leprae. A review with criteria for evaluating recent experimental work</title><title>Bulletin of the World Health Organization</title><addtitle>Bull World Health Organ</addtitle><description>Some criteria are presented to help evaluate papers appearing in the literature claiming successful cultivation of M. leprae either in the absence or in the presence of tissue-cultured cells. Recently, electron microscopic studies have definitely shown M. leprae to belong to the genus Mycobacterium and its division to occur through transverse section. A survey is given of the mycobacterial strains isolated in the last 10 years from leprosy lesions. These strains belong to taxonomically different species and cannot be considered to be M. leprae. No substantiated claim was made concerning the in vitro growth of M. leprae and the application of the tissue culture technique has been equally disappointing. The view is expressed that progress towards the in vitro cultivation of M. leprae can be made only as a result of increased knowledge about the intracellular environment and the metabolic activities of this organism, to be obtained by the application of modern biochemical and histochemical techniques.</description><subject>Animals</subject><subject>Bacteriological Techniques</subject><subject>Cell Division</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Culture Techniques</subject><subject>Ganglia</subject><subject>Haplorhini</subject><subject>Humans</subject><subject>L Forms - isolation & purification</subject><subject>Leprosy - microbiology</subject><subject>Macrophages</subject><subject>Mice</subject><subject>Microscopy, Electron</subject><subject>Mycobacterium - classification</subject><subject>Mycobacterium - growth & development</subject><subject>Mycobacterium - isolation & purification</subject><subject>Mycobacterium leprae - cytology</subject><subject>Mycobacterium leprae - growth & development</subject><subject>Mycobacterium leprae - isolation & purification</subject><subject>Mycobacterium leprae - metabolism</subject><subject>Mycobacterium lepraemurium - growth & development</subject><subject>Mycobacterium lepraemurium - isolation & purification</subject><subject>Mycobacterium lepraemurium - metabolism</subject><subject>Rats</subject><issn>0042-9686</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><recordid>eNpVUEFOwzAQzAFUSuEJSD5xC3Ic14kvSFUFFKmISzlbjrNuDU4cHCelv8cVFYK97K5mdnY0Z8kUY0pSzkp2kVz2_TuOxSmeJBNKMkJzPk38Zgeo866y0CCnkRpsMKMMxrXH9eWgXCVVAG-GBlnovIQ7tEAeRgN7tDdhh5Q3R1wi7TyCUdohnrfbyFHQBgRfXUSbOEqL9s5_XCXnWtoerk99lrw9PmyWq3T9-vS8XKzTjrAspATKrABQms6rQjOWE13ImpKK45LXjLAKFGdQQ5aVJTDKtSakgCJjMJ9jLvNZcv-j2w1VA_XRjJdWdNGM9AfhpBH_kdbsxNaNgtAyxkSjwO1JwLvPAfogGtMrsFa24IZelIQwTDCPxJu_n35fnFLOvwFtRHuc</recordid><startdate>1973</startdate><enddate>1973</enddate><creator>Pattyn, S R</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>1973</creationdate><title>The problem of cultivation of Mycobacterium leprae. 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No substantiated claim was made concerning the in vitro growth of M. leprae and the application of the tissue culture technique has been equally disappointing. The view is expressed that progress towards the in vitro cultivation of M. leprae can be made only as a result of increased knowledge about the intracellular environment and the metabolic activities of this organism, to be obtained by the application of modern biochemical and histochemical techniques.</abstract><cop>Switzerland</cop><pmid>4212439</pmid><tpages>8</tpages></addata></record> |
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| ispartof | Bulletin of the World Health Organization, 1973, Vol.49 (4), p.403-410 |
| issn | 0042-9686 |
| language | eng |
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| source | PubMed Central |
| subjects | Animals Bacteriological Techniques Cell Division Cell Line Cells, Cultured Culture Media Culture Techniques Ganglia Haplorhini Humans L Forms - isolation & purification Leprosy - microbiology Macrophages Mice Microscopy, Electron Mycobacterium - classification Mycobacterium - growth & development Mycobacterium - isolation & purification Mycobacterium leprae - cytology Mycobacterium leprae - growth & development Mycobacterium leprae - isolation & purification Mycobacterium leprae - metabolism Mycobacterium lepraemurium - growth & development Mycobacterium lepraemurium - isolation & purification Mycobacterium lepraemurium - metabolism Rats |
| title | The problem of cultivation of Mycobacterium leprae. A review with criteria for evaluating recent experimental work |
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