Probiotic Lactobacillus acidophilus enhances the immunogenicity of an oral rotavirus vaccine in gnotobiotic pigs

Abstract We evaluated virus-specific B and T cell responses induced by the attenuated Wa (P1A[8]G1) human rotavirus (AttHRV) oral 2-dose vaccine with or without Lactobacillus acidophilus (LA) colonization in neonatal gnotobiotic (Gn) pigs. The AttHRV vaccinated and LA-fed pigs had a significantly hi...

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Bibliographic Details
Published in:Vaccine 2008-07, Vol.26 (29), p.3655-3661
Main Authors: Zhang, Wei, Azevedo, Marli S.P, Wen, Ke, Gonzalez, Ana, Saif, Linda J, Li, Guohua, Yousef, Ahmed E, Yuan, Lijuan
Format: Article
Language:English
Subjects:
Administration, Oral
Allergy and Immunology
Animals
Applied microbiology
B and T cell immune responses to rotavirus vaccine
B-Lymphocytes - immunology
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Germ-Free Life
Gnotobiotic pigs
Human rotavirus
Humans
Ileum - immunology
Immunization, Secondary
Immunoglobulin A - immunology
Immunoglobulin G - immunology
Immunoglobulin M
Immunologic Factors - pharmacology
Interferon-gamma - secretion
Lactobacillus acidophilus
Lactobacillus acidophilus - immunology
Microbiology
Miscellaneous
Neutralization Tests
Probiotic Lactobacillus
Probiotics - pharmacology
Rotavirus Vaccines - administration & dosage
Rotavirus Vaccines - immunology
Spleen - immunology
Swine
T-Lymphocytes - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, Attenuated - administration & dosage
Vaccines, Attenuated - immunology
Virology
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Summary:Abstract We evaluated virus-specific B and T cell responses induced by the attenuated Wa (P1A[8]G1) human rotavirus (AttHRV) oral 2-dose vaccine with or without Lactobacillus acidophilus (LA) colonization in neonatal gnotobiotic (Gn) pigs. The AttHRV vaccinated and LA-fed pigs had a significantly higher magnitude of HRV-specific IFN-γ producing CD8+ T cell responses in ileum and spleen, IgA and IgG antibody-secreting cell responses in ileum, and serum IgM, IgA and IgG antibody and virus neutralizing antibody titers compared to the AttHRV vaccinated pigs without LA colonization. These findings suggest that L. acidophilus has significant immunopotentiating effects and may be used as a safe oral adjuvant for rotavirus vaccines in neonates.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2008.04.070