Localization of myo-inositol-1-phosphate synthase to the endosperm in developing seeds of Arabidopsis

Expression and localization of myo-inositol-1-phosphate synthase (MIPS) in developing seeds of Arabidopsis thaliana was investigated. MIPS is an essential enzyme for production of inositol and inositol phosphates via its circularization of glucose-6-phosphate as the initial step. myo-inositol-6-phos...

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Bibliographic Details
Published in:Journal of experimental botany 2008-08, Vol.59 (11), p.3069-3076
Main Authors: Mitsuhashi, Naoto, Kondo, Maki, Nakaune, Satoru, Ohnishi, Miwa, Hayashi, Makoto, Hara-Nishimura, Ikuko, Richardson, Alan, Fukaki, Hidehiro, Nishimura, Mikio, Mimura, Tetsuro
Format: Article
Language:English
Subjects:
Antibodies
Arabidopsis - embryology
Arabidopsis - enzymology
Arabidopsis - genetics
Arabidopsis thaliana
Biological and medical sciences
Cytosol - enzymology
Embryos
Endosperm
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Gene Expression
Germination and dormancy
Inositol
inositol phosphates
inositol-6-phosphates
Inositols
Microscopy
myo-inositol-1-phosphate synthase
Myo-Inositol-1-Phosphate Synthase - genetics
Myo-Inositol-1-Phosphate Synthase - metabolism
phosphate
phytate
Phytic Acid - biosynthesis
Plant physiology and development
Plants
Research Papers
Rice
Seeds
Seeds - embryology
Seeds - enzymology
Soybeans
Testa
vacuole
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Summary:Expression and localization of myo-inositol-1-phosphate synthase (MIPS) in developing seeds of Arabidopsis thaliana was investigated. MIPS is an essential enzyme for production of inositol and inositol phosphates via its circularization of glucose-6-phosphate as the initial step. myo-inositol-6-phosphate (InsP6 or phytic acid) is the predominant form of phosphorus found in seeds and accumulates as a consequence of MIPS action. Three MIPS genes have been identified in Arabidopsis, all of which were expressed not only in siliques but in both leaves and roots. Immunoelectron microscopy using a MIPS antibody showed that MIPS localizes to the cytosol primarily in the endosperm during seed development and not in the embryo. This is consistent with results obtained using fluorescent microscopy and western blot analysis that showed a similar pattern of localization. However, InsP6, which is the final product of inositol phosphate metabolism, was present mainly in the embryo. This suggests that a complex interaction between the endosperm and embryo occurs during the synthesis and subsequent accumulation of InsP6 in developing seeds of Arabidopsis.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/ern161