Identification of cis-acting sites for condensin loading onto budding yeast chromosomes

Eukaryotic chromosomes reach their stable rod-shaped appearance in mitosis in a reaction dependent on the evolutionarily conserved condensin complex. Little is known about how and where condensin associates with chromosomes. Here, we analyze condensin binding to budding yeast chromosomes using high-...

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Bibliographic Details
Published in:Genes & development 2008-08, Vol.22 (16), p.2215-2227
Main Authors: D'Ambrosio, Claudio, Schmidt, Christine Katrin, Katou, Yuki, Kelly, Gavin, Itoh, Takehiko, Shirahige, Katsuhiko, Uhlmann, Frank
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - physiology
Binding Sites
Cell Cycle
Cell Cycle Proteins
Chromatin Assembly and Disassembly
Chromatin Immunoprecipitation
Chromosomal Proteins, Non-Histone
Chromosomes, Fungal - genetics
Cohesins
DNA-Binding Proteins - physiology
Fungal Proteins - physiology
Mitosis
Multiprotein Complexes - physiology
Research Paper
RNA Polymerase III - metabolism
RNA, Transfer - genetics
Saccharomyces cerevisiae
Saccharomycetales - cytology
Saccharomycetales - genetics
Saccharomycetales - metabolism
Schizosaccharomyces pombe
Transcription Factors, TFIII - physiology
Transcription, Genetic
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Summary:Eukaryotic chromosomes reach their stable rod-shaped appearance in mitosis in a reaction dependent on the evolutionarily conserved condensin complex. Little is known about how and where condensin associates with chromosomes. Here, we analyze condensin binding to budding yeast chromosomes using high-resolution oligonucleotide tiling arrays. Condensin-binding sites coincide with those of the loading factor Scc2/4 of the related cohesin complex. The sites map to tRNA and other genes bound by the RNA polymerase III transcription factor TFIIIC, and ribosomal protein and SNR genes. An ectopic B-box element, recognized by TFIIIC, constitutes a minimal condensin-binding site, and TFIIIC and the Scc2/4 complex promote functional condensin association with chromosomes. A similar pattern of condensin binding is conserved along fission yeast chromosomes. This reveals that TFIIIC-binding sites, including tRNA genes, constitute a hitherto unknown chromosomal feature with important implications for chromosome architecture during both interphase and mitosis.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.1675708