Application of a column selection system and DryLab software for high-performance liquid chromatography method development

This paper describes a strategy for the development of chromatographic methods for drug candidates based upon the use of simple MS compatible mobile phases and optimization of the chromatographic selectivity through variations of the stationary phase and mobile phase pH. The strategy employs an auto...

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Bibliographic Details
Published in:Journal of Chromatography A 2006-07, Vol.1122 (1), p.186-193
Main Authors: Krisko, Ryan M., McLaughlin, Kieran, Koenigbauer, Michael J., Lunte, Craig E.
Format: Article
Language:English
Subjects:
Analysis
Atenolol - analysis
Atenolol - chemistry
Automated method development
Biological and medical sciences
Bupivacaine - analysis
Bupivacaine - chemistry
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Computer Simulation
Drug Stability
DryLab
General pharmacology
HPLC
Hydrogen-Ion Concentration
Mass Spectrometry - methods
Medical sciences
Pharmaceutical Preparations - analysis
Pharmaceutical Preparations - chemistry
Pharmacology. Drug treatments
Reproducibility of Results
Software
Spectrophotometry, Ultraviolet - methods
Stereoisomerism
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Summary:This paper describes a strategy for the development of chromatographic methods for drug candidates based upon the use of simple MS compatible mobile phases and optimization of the chromatographic selectivity through variations of the stationary phase and mobile phase pH. The strategy employs an automated column selection system and a series of HPLC columns, varying in hydrophobicity and silanol activity, in combination with DryLab software to develop chromatographic methods for the separation of mixtures of bupivacaine and its metabolites; acidic, basic, and neutral compounds; and atenolol, nitrendipine, and their degradation products.
ISSN:0021-9673
DOI:10.1016/j.chroma.2006.04.065