Squalene epoxidase, located on chromosome 8q24.1, is upregulated in 8q+ breast cancer and indicates poor clinical outcome in stage I and II disease

Gains of chromosomes 7p and 8q are associated with poor prognosis among oestrogen receptor-positive (ER+) stage I/II breast cancer. To identify transcriptional changes associated with this breast cancer subtype, we applied suppression subtractive hybridisation method to analyse differentially expres...

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Bibliographic Details
Published in:British journal of cancer 2008-09, Vol.99 (5), p.774-780
Main Authors: Helms, M W, Kemming, D, Pospisil, H, Vogt, U, Buerger, H, Korsching, E, Liedtke, C, Schlotter, C M, Wang, A, Chan, S Y, Brandt, B H
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Breast cancer
Breast Neoplasms - enzymology
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Cancer Research
Chromosome Mapping
Chromosomes
Chromosomes, Human, Pair 8
Clinical outcomes
DNA Primers
DNA, Complementary
Drug Resistance
Epidemiology
Gene expression
Gene Expression Profiling
Gynecology
Gynecology. Andrology. Obstetrics
Humans
Mammary gland diseases
Medical prognosis
Medical research
Medical sciences
Metastasis
Molecular Diagnostics
Molecular Medicine
Neoplasm Metastasis
Obstetrics
Oligonucleotide Array Sequence Analysis
Oncology
Patients
Prognosis
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Squalene Monooxygenase - genetics
Survival Analysis
Treatment Outcome
Tumors
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Summary:Gains of chromosomes 7p and 8q are associated with poor prognosis among oestrogen receptor-positive (ER+) stage I/II breast cancer. To identify transcriptional changes associated with this breast cancer subtype, we applied suppression subtractive hybridisation method to analyse differentially expressed genes among six breast tumours with and without chromosomal 7p and 8q gains. Identified mRNAs were validated by real-time RT–PCR in tissue samples obtained from 186 patients with stage I/II breast cancer. Advanced statistical methods were applied to identify associations of mRNA expression with distant metastasis-free survival (DMFS). mRNA expression of the key enzyme of cholesterol biosynthesis, squalene epoxidase (SQLE, chromosomal location 8q24.1), was associated with ER+ 7p+/8q+ breast cancer. Distant metastasis-free survival in stage I/II breast cancer cases was significantly inversely related to SQLE mRNA in multivariate Cox analysis ( P
ISSN:0007-0920
1532-1827
DOI:10.1038/sj.bjc.6604556