Involvement of MAPK activation in chemokine or COX-2 productions by Toxoplasma gondii

This experiment focused on MAPK activation in host cell invasion and replication of T. gondii, as well as the expression of CC chemokines, MCP-1 and MIP-1α, and enzyme, COX-2/prostaglandin E₂(PGE₂) in infected cells via western blot, [³H]-uracil incorporation assay, ELISA and RT-PCR. The phosphoryla...

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Bibliographic Details
Published in:Korean journal of parasitology 2006-09, Vol.44 (3), p.197-207
Main Authors: Kim, J.Y. (Hanyang University College of Medicine, Seoul, Republic of Korea), Ahn, M.H. (Hanyang University College of Medicine, Seoul, Republic of Korea), E-mail: mhahn@hanyang.ac.kr, Song, H.O. (Hanyang University College of Medicine, Seoul, Republic of Korea), Choi, J.H. (Hanyang University College of Medicine, Seoul, Republic of Korea), Ryu, J.S. (Hanyang University College of Medicine, Seoul, Republic of Korea), Min, D.Y. (Hanyang University College of Medicine, Seoul, Republic of Korea), Cho, M.H. (Konkuk University, Seoul, Republic of Korea)
Format: Article
Language:English
Subjects:
Animals
chemokine
Chemokines - biosynthesis
Cyclooxygenase 2 - biosynthesis
cyclooxygenase-2
Enzyme Activation
HeLa Cells
Humans
Macrophages, Peritoneal - enzymology
Macrophages, Peritoneal - immunology
Macrophages, Peritoneal - parasitology
Mice
Mice, Inbred BALB C
mitogen activated protein kinase (MAPK)
Mitogen-Activated Protein Kinases - metabolism
Original
prostaglandin E
Toxoplasma - immunology
Toxoplasma - metabolism
TOXOPLASMA GONDII
Toxoplasmosis - enzymology
Toxoplasmosis - immunology
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Summary:This experiment focused on MAPK activation in host cell invasion and replication of T. gondii, as well as the expression of CC chemokines, MCP-1 and MIP-1α, and enzyme, COX-2/prostaglandin E₂(PGE₂) in infected cells via western blot, [³H]-uracil incorporation assay, ELISA and RT-PCR. The phosphorylation of ERK1/2 and p38 in infected HeLa cells was detected at 1 hr and/or 6 hr postinfection (PI). Tachyzoite proliferation was reduced by p38 or JNK MAPK inhibitors. MCP-1 secretion was enhanced in infected peritoneal macrophages at 6 hr PI.
ISSN:0023-4001
1738-0006
DOI:10.3347/kjp.2006.44.3.197