HP1 Is Distributed Within Distinct Chromatin Domains at Drosophila Telomeres

Telomeric regions in Drosophila are composed of three subdomains. A chromosome cap distinguishes the chromosome end from a DNA double-strand break; an array of retrotransposons, HeT-A, TART, and TAHRE (HTT), maintains telomere length by targeted transposition to chromosome ends; and telomere-associa...

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Bibliographic Details
Published in:Genetics (Austin) 2008-09, Vol.180 (1), p.121-131
Main Authors: Frydrychova, Radmila Capkova, Mason, James M, Archer, Trevor K
Format: Article
Language:English
Subjects:
Animals
Arrays
chromatin
Chromatin - chemistry
Chromatin - genetics
Chromosomal Proteins, Non-Histone - genetics
Chromosomes - ultrastructure
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophila Proteins - genetics
Environmental health
Gene Silencing
Genetics
heterochromatin protein 1
HTT array
Investigations
Micrococcal Nuclease - genetics
Models, Genetic
Mutation
nucleoproteins
Nucleosomes - ultrastructure
Protein Binding
Protein Structure, Tertiary
Proteins
Telomere - ultrastructure
telomere-associated sequence
telomeres
Transcription, Genetic
Transgenes
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Summary:Telomeric regions in Drosophila are composed of three subdomains. A chromosome cap distinguishes the chromosome end from a DNA double-strand break; an array of retrotransposons, HeT-A, TART, and TAHRE (HTT), maintains telomere length by targeted transposition to chromosome ends; and telomere-associated sequence (TAS), which consists of a mosaic of complex repeated sequences, has been identified as a source of gene silencing. Heterochromatin protein 1 (HP1) and HP1-ORC-associated protein (HOAP) are major protein components of the telomere cap in Drosophila and are required for telomere stability. Besides the chromosome cap, HP1 is also localized along the HTT array and in TAS. Mutants for Su(var)205, the gene encoding HP1, have decreased the HP1 level in the HTT array and increased transcription of individual HeT-A elements. This suggests that HP1 levels directly affect HeT-A activity along the HTT array, although they have little or no effect on transcription of a white reporter gene in the HTT. Chromatin immunoprecipitation to identify other heterochromatic proteins indicates that TAS and the HTT array may be distinct from either heterochromatin or euchromatin.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1534/genetics.108.090647