Mix and measure fluorescence screening for selective quadruplex binders

The human genome contains thousands of regions, including that of the telomere, that have the potential to form quadruplex structures. Many of these regions are potential targets for therapeutic intervention. There are many different folding patterns for quadruplex DNAs and the loops exhibit much mo...

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Bibliographic Details
Published in:Nucleic acids research 2008-10, Vol.36 (17), p.e106-e106
Main Authors: Paramasivan, Sattanathan, Bolton, Philip H
Format: Article
Language:English
Subjects:
Benzothiazoles - chemistry
Binding, Competitive
Carbocyanines - chemistry
Circular Dichroism
DNA - chemistry
Fluorescent Dyes - chemistry
G-Quadruplexes
Ligands
Mesoporphyrins - chemistry
Methods Online
Spectrometry, Fluorescence
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Summary:The human genome contains thousands of regions, including that of the telomere, that have the potential to form quadruplex structures. Many of these regions are potential targets for therapeutic intervention. There are many different folding patterns for quadruplex DNAs and the loops exhibit much more variation than do the quartets. The successful targeting of a particular quadruplex structure requires distinguishing that structure from all of the other quadruplex structures that may be present. A mix and measure fluorescent screening method has been developed, that utilizes multiple reporter molecules that bind to different features of quadruplex DNA. The reporter molecules are used in combination with DNAs that have a variety of quadruplex structures. The screening is based on observing the increase or decrease in the fluorescence of the reporter molecules. The selectivity of a set of test molecules has been determined by this approach.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkn487