Loading…

Human TSLP directly enhances expansion of CD8⁺ T cells

Human thymic stromal lymphopoietin (TSLP) promotes CD4⁺ T-cell proliferation both directly and indirectly through dendritic cell (DC) activation. Although human TSLP-activated DCs induce CD8⁺ T-cell proliferation, it is not clear whether TSLP acts directly on CD8⁺ T cells. In this study, we show tha...

Full description

Saved in:
Bibliographic Details
Published in:Clinical and experimental immunology 2008-10, Vol.154 (1), p.98-106
Main Authors: Akamatsu, T, Watanabe, N, Kido, M, Saga, K, Tanaka, J, Kuzushima, K, Nishio, A, Chiba, T
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human thymic stromal lymphopoietin (TSLP) promotes CD4⁺ T-cell proliferation both directly and indirectly through dendritic cell (DC) activation. Although human TSLP-activated DCs induce CD8⁺ T-cell proliferation, it is not clear whether TSLP acts directly on CD8⁺ T cells. In this study, we show that human CD8⁺ T cells activated by T-cell receptor stimulation expressed TSLP receptor (TSLPR), and that TSLP directly enhanced proliferation of activated CD8⁺ T cells. Although non-stimulated human CD8⁺ T cells from peripheral blood did not express TSLPR, CD8⁺ T cells activated by anti-CD3 plus anti-CD28 did express TSLPR. After T-cell receptor stimulation, TSLP directly enhanced the expansion of activated CD8⁺ T cells. Interestingly, using monocyte-derived DCs pulsed with a cytomegalovirus (CMV)-specific pp65 peptide, we found that although interleukin-2 allowed expansion of both CMV-specific and non-specific CD8⁺ T cells, TSLP induced expansion of only CMV-specific CD8⁺ T cells. These results suggest that human TSLP directly enhances expansion of CD8⁺ T cells and that the direct and indirect action of TSLP on expansion of target antigen-specific CD8⁺ T cells may be beneficial to adoptive cell transfer immunotherapy.
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.2008.03731.x