Agrobacterium tumefaciens-mediated genetic transformation of the phytopathogenic fungus Penicillium digitatum

Agrobacterium tumefaciens-mediated transformation （ATMT） system was assessed for conducting insertional mutagenesis in Penicillium digitatum, a major fungal pathogen infecting post-harvest citrus fruits. A transformation efficiency of up to 60 transformants per 106 conidia was achieved by this syste...

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Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2008-10, Vol.9 (10), p.823-828
Main Authors: Wang, Ji-ye, Li, Hong-ye
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens - genetics
ATMT
Base Sequence
Biomedical and Life Sciences
Biomedicine
Citrus - microbiology
DNA Primers - genetics
DNA, Bacterial - genetics
DNA, Fungal - genetics
DNA, Recombinant - genetics
Drug Resistance, Fungal - genetics
Genomes
Hygromycin B - pharmacology
Molecular Sequence Data
Mutagenesis
Mutagenesis, Insertional
Mutation
Penicillium - drug effects
Penicillium - genetics
Penicillium - pathogenicity
Plant Diseases - microbiology
Plasmids - genetics
Transformation, Genetic
基因突变
潮霉素
青霉菌
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Summary:Agrobacterium tumefaciens-mediated transformation （ATMT） system was assessed for conducting insertional mutagenesis in Penicillium digitatum, a major fungal pathogen infecting post-harvest citrus fruits. A transformation efficiency of up to 60 transformants per 106 conidia was achieved by this system. The integration of the hph gene into the fungal genome was verified by polymerase chain reaction （PCR） amplification and sequencing. These transformants tested were also shown to be mitotically stable. Southern blot analysis of 14 randomly selected transformants showed that the hph gene was randomly integrated as single copy into the fungal genome of P. digitatum. Thus, we conclude that ATMT of P. digitatum could be used as an alternatively practical genetic tool for conducting insertional mutagenesis in P. digitatum to study functional genomics.
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B0860006