Expression of carboxylesterase and lipase genes in rat liver cell-types

Approximately 80% of the body vitamin A is stored in liver stellate cells with in the lipid droplets as retinyl esters. In low vitamin A status or after liver injury, stellate cells are depleted of the stored retinyl esters by their hydrolysis to retinol. However, the identity of retinyl ester hydro...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-09, Vol.374 (3), p.460-464
Main Authors: Mello, Tommaso, Nakatsuka, Alice, Fears, Sharry, Davis, Wilhelmina, Tsukamoto, Hidekazu, Bosron, William F., Sanghani, Sonal P.
Format: Article
Language:English
Subjects:
Adipocytes triglyceride lipase
Animals
Carboxylesterases
Carboxylic Ester Hydrolases - genetics
Cell Separation
Cells, Cultured
Endothelial Cells - enzymology
Gene Expression
Hepatic stellate cells
Hepatocytes - enzymology
Kupffer Cells - enzymology
Lipase - genetics
Lipoprotein lipase
Lipoprotein Lipase - genetics
Liver - cytology
Liver - enzymology
Polymerase Chain Reaction
Rats
Real-time PCR
Retinyl palmitate
Vitamin A
Vitamin A - metabolism
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Summary:Approximately 80% of the body vitamin A is stored in liver stellate cells with in the lipid droplets as retinyl esters. In low vitamin A status or after liver injury, stellate cells are depleted of the stored retinyl esters by their hydrolysis to retinol. However, the identity of retinyl ester hydrolase(s) expressed in stellate cells is unknown. The expression of carboxylesterase and lipase genes in purified liver cell-types was investigated by real-time PCR. We found that six carboxylesterase and hepatic lipase genes were expressed in hepatocytes. Adipose triglyceride lipase was expressed in Kupffer cells, stellate cells and endothelial cells. Lipoprotein lipase expression was detected in Kupffer cells and stellate cells. As a function of stellate cell activation, expression of adipose triglyceride lipase decreased by twofold and lipoprotein lipase increased by 32-fold suggesting that it may play a role in retinol ester hydrolysis during stellate cell activation.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.07.024