Conservation of inter-protein binding sites in RUSH and RFBP, an ATP11B isoform

Isoforms of RUSH interact with a RING-finger binding protein (RFBP), which is a splice variant of the Type IV P-type ATPase, ATP11B. Splice arrays and RT-PCR showed that although most splice variants in RUSH and ATP11B are conserved in human and rabbit, the RFBP isoform is specific to rabbit. Intera...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 2008-09, Vol.292 (1), p.79-86
Main Authors: Hewetson, Aveline, Wright-Pastusek, Amber E., Helmer, Rebecca A., Wesley, Kerrie A., Chilton, Beverly S.
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - chemistry
Adenosine Triphosphatases - metabolism
Alternative splicing
Amino Acid Sequence
Animals
ATP-Binding Cassette Transporters - chemistry
ATP-Binding Cassette Transporters - genetics
ATP11B
ATPase Inhibitory Protein
Binding Sites
Carrier Proteins - chemistry
Carrier Proteins - metabolism
CLIPS-technology
Conserved Sequence
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - metabolism
Exons - genetics
Female
Humans
Immunoprecipitation
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Peptides - chemistry
Protein Binding
Protein Conformation
Protein Isoforms
Proteins - chemistry
Proteins - metabolism
Rabbits
Reverse Transcriptase Polymerase Chain Reaction
RING-finger
RNA Splicing
RUSH
SMARCA3/HLTF
Transcription Factors - chemistry
Transcription Factors - metabolism
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Summary:Isoforms of RUSH interact with a RING-finger binding protein (RFBP), which is a splice variant of the Type IV P-type ATPase, ATP11B. Splice arrays and RT-PCR showed that although most splice variants in RUSH and ATP11B are conserved in human and rabbit, the RFBP isoform is specific to rabbit. Interactions between the discontinuous PVITHC–HAKCPL sequence in the RING-domain of RUSH and the KVIRLIKIS sequence in the catalytic loop of RFBP were first identified with pull-down assays. Fine mapping involved probing CLIPS-constrained RING peptides with GST-tagged KVIRLIKIS. When the companion site in RFBP was fine mapped by replacement analysis with MBP-tagged RING, a four-fold increase in binding was noted for the KVIRLDKIS mutant. Direct comparison of splicing events in the RUSH and ATP11B genes between human and rabbit shows high structural stability in these protein interactions sites, which are 100% conserved in all mammalian orthologs.
ISSN:0303-7207
1872-8057
DOI:10.1016/j.mce.2008.05.007