Ethylene- and pathogen-inducible Arabidopsis acyl-CoA-binding protein 4 interacts with an ethylene-responsive element binding protein

• Published in: Journal of experimental botany 2008-10, Vol.59 (14), p.3997-4006
• Main Authors: Li, Hong-Ye, Xiao, Shi, Chye, Mee-Len
• Format: Article
• Language: English
• Subjects: Acyl-CoA-binding protein; Antibodies; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis - microbiology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Biological and medical sciences; Botrytis - physiology; Botrytis cinerea; Carrier proteins; Carrier Proteins - genetics; Carrier Proteins - metabolism; Complementary DNA; Cytosol; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; ethylene; Ethylenes - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression; Genes; Host-Pathogen Interactions; Molecular interactions; pathogen; Plant Diseases - microbiology; Plant Proteins - genetics; Plant Proteins - metabolism; Plants; Plasmids; Protein Binding; protein–protein interaction; Research Papers; RNA; Two-Hybrid System Techniques; Yeasts
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/ern241

Six genes encode proteins with acyl-CoA-binding domains in Arabidopsis thaliana. They are the small 10-kDa cytosolic acyl-CoA-binding protein (ACBP), membrane-associated ACBP1 and ACBP2, extracellularly-targeted ACBP3, and kelch-motif containing ACBP4 and ACBP5. Here, the interaction of ACBP4 with an A. thaliana ethylene-responsive element binding protein (AtEBP), identified in a yeast two-hybrid screen, was confirmed by co-immunoprecipitation. The subcellular localization of ACBP4 and AtEBP, was addressed using an ACBP4:DsRed red fluorescent protein fusion and a green fluorescent protein (GFP):AtEBP fusion. Transient expression of these autofluoresence-tagged proteins in agroinfiltrated tobacco leaves, followed by confocal laser scanning microscopy, indicated their co-localization predominantly at the cytosol which was confirmed by FRET analysis. Immuno-electron microscopy on Arabidopsis sections not only localized ACBP4 to the cytosol but also to the periphery of the nucleus upon closer examination, perhaps as a result of its interaction with AtEBP. Furthermore, the expression of ACBP4 and AtEBP in Northern blot analyses was induced by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, methyl jasmonate treatments, and Botrytis cinerea infection, suggesting that the interaction of ACBP4 and AtEBP may be related to AtEBP-mediated defence possibly via ethylene and/or jasmonate signalling.