Incorporation of a Fluorescent Compound by Live Heterodera glycines

The incorporation of fluorescein isothiocyanate (FITC) by J2 of Heterodera glycines, the soybean cyst nematode, and the resulting effects on fitness were determined. Live soybean cyst nematode J2 incubated in FITC fluoresced, primarily in the intestinal region, beyond auto-fluorescence. Dissection o...

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Bibliographic Details
Published in:Journal of nematology 2007-03, Vol.39 (1), p.43-49
Main Authors: Schroeder, N.E, MacGuidwin, A.E
Format: Article
Language:English
Subjects:
biomarkers
Contributed Paper
cyst nematodes
egg hatchability
fluorescein isothiocyanate
fluorescence
fluorescent dyes
fluorescent labeling
Heterodera glycines
isothiocyanates
nematode fitness
nematode larvae
pathogenicity
rhodamine B isothiocyanate
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Summary:The incorporation of fluorescein isothiocyanate (FITC) by J2 of Heterodera glycines, the soybean cyst nematode, and the resulting effects on fitness were determined. Live soybean cyst nematode J2 incubated in FITC fluoresced, primarily in the intestinal region, beyond auto-fluorescence. Dissection of animals, as well as fluorescence-quenching techniques, indicated that FITC was not simply bound to the cuticle. FITC was also found to cross the egg shell. Fluorescence increased in relation to FITC concentration and incubation time. Nematodes incubated in FITC remained active and did not lose their fluorescence even after two weeks at room temperature. Fluorescence of nematodes was not stable through development. Males which developed from fluorescent juveniles did not retain the stain. Both FITC and the DMF solvent reduced the hatching rate. However, those individuals that successfully hatched remained viable and able to infect roots. Incorporation of FITC was found to occur in three other genera of nematodes. Rhodamine B isothiocyanate was also found to be incorporated by H. glycines.
ISSN:0022-300X
2640-396X