Nanofluidic Biosensing for β-Amyloid Detection Using Surface Enhanced Raman Spectroscopy

• Published in: Nano letters 2008-06, Vol.8 (6), p.1729-1735
• Main Authors: Chou, I-Hsien, Benford, Melodie, Beier, Hope T, Coté, Gerard L, Wang, Miao, Jing, Nan, Kameoka, Jun, Good, Theresa A
• Format: Article
• Language: English
• Subjects: Amyloid beta-Peptides - analysis; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biotechnology; Cross-disciplinary physics: materials science; rheology; Equipment Design; Equipment Failure Analysis; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Materials science; Methods of nanofabrication; Methods. Procedures. Technologies; Microfluidic Analytical Techniques - instrumentation; Nanotechnology - instrumentation; Nanotechnology - methods; Others; Physics; Self-assembly; Spectrum Analysis, Raman - instrumentation; Spectrum Analysis, Raman - methods; Surface Plasmon Resonance - instrumentation; Surface Plasmon Resonance - methods; Various methods and equipments
• ISSN: 1530-6984; 1530-6992
• DOI: 10.1021/nl0808132

Trace detection of the conformational transition of β-amyloid peptide (Aβ) from a predominantly α-helical structure to β-sheet could have a large impact in understanding and diagnosing Alzheimer’s disease. We demonstrate how a novel nanofluidic biosensor using a controlled, reproducible surface enhanced Raman spectroscopy active site was developed to observe Aβ in different conformational states during the Aβ self-assembly process as well as to distinguish Aβ from confounder proteins commonly found in cerebral spinal fluid.