Elucidation of the mode of interaction in the UP1-telomerase RNA-telomeric DNA ternary complex which serves to recruit telomerase to telomeric DNA and to enhance the telomerase activity

We found that UP1, a proteolytic product of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), both enhances and represses the telomerase activity. The formation of the UP1-telomerase RNA-telomeric DNA ternary complex was revealed by a gel retardation experiment. The interactions in the ternary...

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Bibliographic Details
Published in:Nucleic acids research 2008-12, Vol.36 (21), p.6816-6824
Main Authors: Nagata, Takashi, Takada, Yusuke, Ono, Asami, Nagata, Kayoko, Konishi, Yuki, Nukina, Takeshi, Ono, Manami, Matsugami, Akimasa, Furukawa, Ayako, Fujimoto, Natsuki, Fukuda, Hirokazu, Nakagama, Hitoshi, Katahira, Masato
Format: Article
Language:English
Subjects:
DNA - chemistry
DNA - metabolism
Electrophoretic Mobility Shift Assay
HeLa Cells
Heterogeneous Nuclear Ribonucleoprotein A1
Heterogeneous-Nuclear Ribonucleoprotein Group A-B - chemistry
Heterogeneous-Nuclear Ribonucleoprotein Group A-B - metabolism
Humans
Nuclear Magnetic Resonance, Biomolecular
Protein Structure, Secondary
RNA - chemistry
RNA - metabolism
Structural Biology
Telomerase - chemistry
Telomerase - metabolism
Telomere - chemistry
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Summary:We found that UP1, a proteolytic product of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), both enhances and represses the telomerase activity. The formation of the UP1-telomerase RNA-telomeric DNA ternary complex was revealed by a gel retardation experiment. The interactions in the ternary and binary complexes were elucidated by NMR. UP1 has two nucleic acid-binding domains, BD1 and BD2. In the UP1-telomerase RNA binary complex, both BD1 and BD2 interact with telomerase RNA. Interestingly, when telomeric DNA was added to the binary complex, telomeric DNA bound to BD1 in place of telomerase RNA. Thus, BD1 basically binds to telomeric DNA, while BD2 mainly binds to telomerase RNA, which resulted in the formation of the ternary complex. Here, UP1 bridges telomerase and telomeric DNA. It is supposed that UP1/hnRNP A1 serves to recruit telomerase to telomeric DNA through the formation of the ternary complex. A model has been proposed for how hnRNP A1/UP1 contributes to enhancement of the telomerase activity through recruitment and unfolding of the quadruplex of telomeric DNA.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkn767