Control of Precerebellar Neuron Development by Olig3 bHLH Transcription Factor

The rhombic lip (RL) is the neuroepithelium immediately adjacent to the roof plate of the fourth ventricle, and it gives rise to various brainstem and cerebellar cell types. Our study shows that the bHLH (basic helix-loop-helix) transcription factor Olig3 is expressed in the progenitors of RL, and a...

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Bibliographic Details
Published in:The Journal of neuroscience 2008-10, Vol.28 (40), p.10124-10133
Main Authors: Liu, Zijing, Li, Hong, Hu, Xuemei, Yu, Ling, Liu, Hongbin, Han, Ruifa, Colella, Rita, Mower, George D, Chen, Yiping, Qiu, Mengsheng
Format: Article
Language:English
Subjects:
Animals
Basic Helix-Loop-Helix Transcription Factors - biosynthesis
Basic Helix-Loop-Helix Transcription Factors - deficiency
Basic Helix-Loop-Helix Transcription Factors - physiology
Cell Differentiation - physiology
Cerebellum - cytology
Cerebellum - embryology
Cerebellum - physiology
Embryonic Stem Cells - cytology
Embryonic Stem Cells - physiology
Gene Expression Regulation, Developmental - physiology
Mice
Mice, Knockout
Neurons - cytology
Neurons - physiology
Rhombencephalon - cytology
Rhombencephalon - embryology
Rhombencephalon - physiology
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Summary:The rhombic lip (RL) is the neuroepithelium immediately adjacent to the roof plate of the fourth ventricle, and it gives rise to various brainstem and cerebellar cell types. Our study shows that the bHLH (basic helix-loop-helix) transcription factor Olig3 is expressed in the progenitors of RL, and ablation of Olig3 significantly affects the development of RL. In Olig3-/- caudal RL, the expression level of Math1 in the dorsal interneuron 1 (dI1) domain is reduced, and the formation of four mossy-fiber nuclei is compromised; dI2-dI3 neurons are misspecified to dI4 interneurons, and the climbing-fiber neurons (inferior olive nucleus) are completely lost. In addition, the formation of brainstem (nor)adrenergic centers and first-order relay visceral sensory neurons is also dependent on Olig3. Therefore, Olig3 plays an important role in the fate specification and differentiation of caudal RL-derived neurons.
ISSN:0270-6474
1529-2401
DOI:10.1523/JNEUROSCI.3769-08.2008