Common patterns of bcl-2 family gene expression in two traumatic brain injury models

Cell death/survival following traumatic brain injury (TBI) may be a result of alterations in the intracellular ratio of death and survival factors. Bcl-2 family genes mediate both cell survival and the initiation of cell death. Using lysate RNase protection assays, mRNA expression of the anti-cell d...

Full description

Saved in:
Bibliographic Details
Published in:Neurotoxicity research 2004, Vol.6 (4), p.333-342
Main Authors: Strauss, Kenneth I, Narayan, Raj K, Raghupathi, Ramesh
Format: Article
Language:English
Subjects:
Animals
bcl-2-Associated X Protein
bcl-X Protein
Brain Injuries - genetics
Brain Injuries - metabolism
Disease Models, Animal
Gene Expression Regulation - physiology
Male
Multigene Family
Proto-Oncogene Proteins c-bcl-2 - biosynthesis
Proto-Oncogene Proteins c-bcl-2 - genetics
Rats
Rats, Sprague-Dawley
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cell death/survival following traumatic brain injury (TBI) may be a result of alterations in the intracellular ratio of death and survival factors. Bcl-2 family genes mediate both cell survival and the initiation of cell death. Using lysate RNase protection assays, mRNA expression of the anti-cell death genes Bcl-2 and Bcl-xL, and the pro-cell death gene Bax, was evaluated following experimental brain injuries in adult male Sprague-Dawley rats. Both the lateral fluid-percussion (LFP) and the lateral controlled cortical impact (LCI) models of TBI showed similar patterns of gene expression. Anti-cell death bcl-2 and bcl-xL mRNAs were attenuated early and tended to remain depressed for at least 3 days after injury in the cortex and hippocampus ipsilateral to injury. Pro-cell death bax mRNA was elevated in these areas, usually following the decrease in anti-cell death genes. These common patterns of gene expression suggest an important role for Bcl-2 genes in cell death and survival in the injured brain. Understanding the regulation of these genes may facilitate the development of new therapeutic strategies for a condition that currently has no proven pharmacologic treatments.
ISSN:1029-8428
1476-3524
DOI:10.1007/BF03033444