Secretion of Insulinotropic Proteins by Commensal Bacteria: Rewiring the Gut To Treat Diabetes

Here, we show that commensal bacteria can stimulate intestinal epithelial cells to secrete insulin in response to glucose. Commensal strains were engineered to secrete the insulinotropic proteins GLP-1 and PDX-1. Epithelia stimulated by engineered strains and glucose secreted up to 1 ng ml⁻¹ of insu...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2008-12, Vol.74 (23), p.7437-7438
Main Authors: Duan, Faping, Curtis, Katherine L, March, John C
Format: Article
Language:English
Subjects:
Bacteria
Bacterial proteins
Biological and medical sciences
Caco-2 Cells
Cells
Diabetes
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Escherichia coli - genetics
Escherichia coli - metabolism
Fundamental and applied biological sciences. Psychology
Glucagon-Like Peptide 1 - biosynthesis
Glucagon-Like Peptide 1 - genetics
Glucose
Homeodomain Proteins - biosynthesis
Homeodomain Proteins - genetics
Humans
Incretins - biosynthesis
Incretins - genetics
Insulin
Insulin - metabolism
Insulin Secretion
Microbiology
Physiology and Biotechnology
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Trans-Activators - biosynthesis
Trans-Activators - genetics
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Summary:Here, we show that commensal bacteria can stimulate intestinal epithelial cells to secrete insulin in response to glucose. Commensal strains were engineered to secrete the insulinotropic proteins GLP-1 and PDX-1. Epithelia stimulated by engineered strains and glucose secreted up to 1 ng ml⁻¹ of insulin with no significant background secretion.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.01019-08